Cytotoxic Activity of against SH-SY5Y Neuroblastoma Cells

Abstract

Effects of essential oils extracted from Dunaliella salina against SH-SY5Y human neuroblastoma cells were investigated in this study. GC-MS analysis was used for determining the composition of essential oils found in Dunaliella salina extracts. All concentrations of Dunaliella salina extract against SH-SY5Y human neuroblastoma cells were significantly more cytotoxic than ECV304 human endothelial cells used as a control. Fifthy compounds were detected in the essential oils of Dunaliella salina, and five major compounds were predominantly found as follows:octadecanoic acid, methyl ester (27.43 %), hexadecanoic acid, methyl ester (Cas) methyl palmitate (24.82 %), 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (7.39 %), octadecanoic acid (5.03 %), pentadecanoic acid (3.60%). The cytotoxic activity of Dunaliella salina extract against SH-SY5Y human neuroblastoma cells was probably due to the high contents of octadecanoic acid and hexadecanoic acid. Furthermore, experimental values indicate that the extract would have some proliferative effect on ECV 304 cells in a dose-dependent manner between 5 and 0.25 μg/ml. These results suggest that Dunaliella salina may have anticancer potential against human neuroblastoma cells