CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
草鱼(AG)微卫星标记克隆及特征分析
Authors
廖兰杰
朱作言
+6 more
杜富宽
江遥
汪亚平
石米娟
连灏
黄容
Publication date
1 January 2012
Publisher
Abstract
报道了一种简便高效克隆微卫星序列的方法。这一方法的实施步骤包括小片段基因组文库构建、三螺旋捕获、磁珠分离和PCR扫描。草鱼AG重复文库的冗余率为7.2%,富集效率为60.25倍,PCR扫描的准确率达100%,采用这一方法克隆到的AG重复序列中完美型、非完美型和混合型的比例分别为66.87%、17.17%和15.96%,不间断重复序列长度大于30 bp的微卫星座位占51%。34个微卫星座位中有25个表现出多态性,PIC值0.50—0.91,进一步分析表明AG重复序列的多态信息含量(PIC)与不间断重复序列长度相关,不间断重复序列长度大于30 bp的座位表现出丰富的多态性。较高的富集率和有效引物获得率证明此方法在分离草鱼微卫星中的成功应用,并将为草鱼养殖品系的优化、遗传多样性的检测及遗传图谱的构建等打下基础
Similar works
Full text
Available Versions
Institute of Hydrobiology, Chinese Academy Of Sciences
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:ir.ihb.ac.cn:342005/42617
Last time updated on 23/01/2021