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鲤鱼(Cyprinus carpio L.)GH受体的分子克隆及其两种转录子的发现
Authors
孙晓凤
朱作言
+3 more
汪亚平
胡炜
郭琼林
Publication date
1 January 2006
Publisher
Abstract
应用反转录-PCR及RACE技术,从二龄鲤鱼肝组织中克隆了生长激素受体(GHR)的全长基因,其开放阅读框编码602个氨基酸,其中包括244个氨基酸的胞外激素结合结构域,24个氨基酸的跨膜区域和334个氨基酸的胞内信号传导区域.序列分析表明:无论在基因水平还是蛋白质水平,鲤鱼GHR与鲫鱼GHR均具有较高的同源性.用一对基因特异性引物在研究二龄鲤鱼GHR的组织分布时发现:肝与其他组织的扩增产物大小不一致(肝组织中的约小100 bp),测序结果以及基因组PCR表明这是由于一个97 bp的内含子选择性剪切造成的
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Institute of Hydrobiology, Chinese Academy Of Sciences
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oai:ir.ihb.ac.cn:342005/39812
Last time updated on 23/01/2021