The Heavy Metal Deposit Visualization on Organ Tissues after Slaughtering on Beef Productio

Adnyane, I Ketut Mudite

Pamungkasari, Yohana Paula Prihatmi

Winarto, Adi

English

Media Veteriner

Proceeding of the 5th Congress of Asian Association of Veterinary Anatomists Bali- INDONESIA, February 11-13th, 2015  54  AH-19  The Heavy Metal Deposit Visualization on Organ Tissues after Slaughtering on Beef Production  Adi Winarto1,*, I Ketut Mudite Adnyane2,  and Yohana Paula Prihatmi Pamungkasari3  1,2 Laboratory of Histology, Department  of Anatomy, Physiology and Pharmacology,  Faculty of Veterinary Medicine, Bogor Agricultural University 3 Citra Pet & Vet Ruko City. Walk 6 No.12. Citragrand, Cibubur-Bekasi *Corresponding author: katanablack704@gmail.com  Keywords:  lead, heavy metal, cow’s tissue, AAS, Rhodizonate  INTRODUCTION  Currently, heavy metal especially lied (Pb) is highly potential in polluting environment that may harmful to animal productions and human health because of its accumulation (Harteman 2011).  The lied accumulation  effect to the risk organs gating wors by increasing deposit number in the tissues target. Since beef is one of animal protein resorces of human meal, it is important to consider thelied accumulation effect to the human health (Suyanto et al. 2010).Over Pb accumulation in vital organs has a toxic effect to the individu (D’Mello 2003). This study was aimed to visualized lead deposit at the muscle, liver and kidney tissues and to examine the lead levels in evaluated tissues.  MATERIALS AND METHODS The tissue samples were collected from slaughter houses surrounds Bogor. Tissue samples were divided into 2 parts, one part was fixed in 4 % paraformaldehyde for histological method, which lied was visualized by Rhodizonate (Kiernan 1990), and the other part was prepared for lied value level analysis using Atomic Absorbent Spectrophotometer.    RESULTS AND DISCUSSION The result showed that the accumulation lied in muscle was scatter in outer connective tissue rather than in muscle cell.  In the liver, lied accumulation was found in hepatocyte at the peripheral area of lobules. While in the kidney, lead was mainly deposited in renal tubule, although slightly also found in glomerular area.  Further evaluation using AAS indicated that all evaluated organ samples showed an exited value from recommended maximum number approved by Indonesian government (SNI 7387:2009). Those lead values levels in the muscle, liver, and kidneys tissues, respectively were as followed 1.24±0.33 ppm, 1.59±0.61 ppm, 1.11±0.20 ppm. The result indicated that excluded the outer muscle connective tissue in beef production will reduce a risk in accumulating lead deposit, and consuming liver and kidney was potentially catch lead from meal, which harmful to the human health. More over these data may indicate how important the regular control on lead pollutant to improve beef quality.  CONCLUSION It can be conclude that both approach giving an easy guidance in understanding the risk of getting lead through the “meat” production. The histochemical data indicated that there may get a hepatocyte function failure risk and get a renal tubular damage when the lead accumulation was there.  REFERENCES 1. D’Mello JPF. 2003. Food SafetyContaminantsandToxins. London: CABI Publishing. 2. Harteman E. 2011. Dampak kandungan logam berat terhadap kemunculan polimorfisme ikan badukang (Arius maculatus Fis&Bian) dan sembilang (Plotosus canius Web&Bia) di muara Proceeding of the 5th Congress of Asian Association of Veterinary Anatomists Bali- INDONESIA, February 12-13th, 2015   55  Sungai Kahayan serta Katingan, Kalimantan Tengah[disertasi]. Bogor (ID): Institut Pertanian Bogor. 268 hlm. 3. Kiernan JA. 1990. Histological and HistochemicalMethods. 2nd ed. Canada: Pergamen Pr. 4. [SNI] Standar Nasional Indonesia 7387:2009. 2009. Batas maksimum cemaran logam berat dalam pangan. Badan Standarisasi Nasional.  

AH-19 The Heavy Metal Deposit Visualization on Organ Tissues after Slaughtering on Beef Production

Scientific Journals of Bogor Agricultural University

http://journal.ipb.ac.id/index.php/veteriner/article/download/18689/13191

AH-19 The Heavy Metal Deposit Visualization on Organ Tissues after Slaughtering on Beef Production

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