The 8th Conference of Asian International Association of Dental Traumatology (AADT) ; November 17-18, 2017 ; Mahidol University, Faculty of Dentistry, Bangkok, THAILAND

Okafuji Norimasa

application/pdfThe 8th Conference of Asian International Association of Dental Traumatology (AADT) ; November 17-18, 2017 ; Mahidol University, Faculty of Dentistry, Bangkok, THAILAND.conference pape

Okafuji, Norimasa

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Matsumoto Dental University Repository

28AADTSpecial lectureProfessor Norimasa OkafujiDepartment of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, Nakano, JapanSpecial lectureCurriculum Vitae1987  D.D.S., Graduate of Matsumoto Dental University,  Japan1996  Ph. D, title by School of Dentistry, Aichi-Gakuin  University, Japan2002  Associate Professor, Department of Orthodontics,  Matsumoto Dental University 2002.12.- Research Fellow of Department of Orthodontics,2003.11.  University of Washington, Seattle, Washington,  USA2006  Specialist appointed by Japanese Orthodontics  Society (No.136)2008  Certified physician of Japan Association of Dental  Traumatology (No.67)2007 Professor, School of Dentistry, Matsumoto Dental  University Professor, Department of Hard Tissue Research,  Graduate School of Oral Medicine, Matsumoto  Dental UniversityThe 8th Conference of Asian International Association of Dental Traumatology (AADT)29AADTSpecial lectureSL-6Bone Marrow Stromal Cells Recovering the Periodontal Ligament in Mouse Experimental Occlusal Trauma Model It is known that traumatic occlusion in the presence of periodontal tissues along with plaque-induced inflammation may have an important contribu- tory role in the progression of periodontal disease. The perio- dontal ligament and histopa- thology-like consideration is mostly experimental of rats, macaque monkeys and beagle dogs as seen in animal experiments performed so far. However, the report did not find a focus point at cytological kinetics of periodontal ligament due to excessive occlusal loading. Thus, we focused the cytological kinetics in the periodontal tissues by excessive occlusal loading. In this study, we established an experimental model of occlusal trauma in mice, and analyzed histopathological Keywords:  GFP, Ki67, Mouse, Occlusal trauma, Periodontal  ligamentand immunohistochemical changes of cytological kinetics in periodontal ligament of the lower left first molar. Using a model of experimental occlusal trauma in mice, we investigated cytological kinetics of perio- dontal ligament by means of histopathological, immunohisto- chemical, and photographical analysis methods. Periodontal ligament cells at furcation areas of molar teeth in the experimental group on day 4 showed a proliferation tendency of perio- dontal ligament cells. The cells with a round-shaped nucleus deeply stained the hematoxylin and increased within the day 4 specimens. Ki67 positive nuclei showed prominent increase in the group on days 4 and 7. Green Fluorescent Protein (GFP) positivity also revealed cell movement but was slightly slow compared to Ki67. It indicated that restoration of mechanism seemed conspicuous by osteo-clasts and macrophages from bone-marrow-derived cells for the periodontal ligament at the furcation area. It was suggested that the remodeling of periodontal ligament with cell acceleration was evoked from the experiment for the group on day 4 and after day 7. Periodontal ligament at the furcation area of the molar teeth in this experimental model recovered using the cells in situ and the bone-marrow-derived cells.

Bone Marrow Stromal Cells Recovering the Periodontal Ligament in Mouse Experimental Occlusal Trauma Model

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Bone Marrow Stromal Cells Recovering the Periodontal Ligament in Mouse Experimental Occlusal Trauma Model

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