Asako Matsui

Fuxiang Ye

Hiroki Kaneko

Hiroko Terasaki

Kei Takayama

Shiang-Jyi Hwang

Shu Kachi

Tadasu Sugita

Yosuke Nagasaka

&lt;b&gt;&lt;i&gt;Purpose:&lt;/i&gt;&lt;/b&gt; Amyloid-β (Aβ) is a 36- to 43-amino-acid peptide that is a constituent of drusen, and it has been demonstrated to upregulate vascular endothelial growth factor (VEGF) expression by retinal pigment epithelial (RPE) cells. This study aimed to determine whether 4-phenylbutyl phosphonylacetate (PBA), a known endoplasmic reticulum (ER) stress inhibitor, can reduce Aβ-induced expression of VEGF in RPE cells. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Aβ was added to the medium of regularly cultured or polarized ARPE-19 cells, a human RPE cell line, with or without PBA. The levels of VEGF and ER stress markers, namely GRP78/Bip, cleaved caspases 4 and 12 and GADD153/C-EBP homologous protein, were determined by enzyme-linked immunoassay, immunocytochemistry and Western blotting. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; Exposure of ARPE-19 cells to Aβ induced GRP78/Bip expression and activated caspases 4 and 12; however, their expression was decreased by simultaneous exposure to PBA. Aβ increased the expression of VEGF both in regularly cultured and polarized ARPE-19 cells, but it was suppressed by PBA. PBA did not cause RPE cell apoptosis. &lt;b&gt;&lt;i&gt;Conclusion:&lt;/i&gt;&lt;/b&gt; Aβ has been suggested to be involved in the development of age-related macular degeneration; therefore, our findings suggest that drugs that target ER stress should be considered for the treatment of age-related macular degeneration.</jats:p

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Ophthalmic Research

Expression of Vascular Endothelial Growth Factor by Retinal Pigment Epithelial Cells Induced by Amyloid-β Is Depressed by an Endoplasmic Reticulum Stress Inhibitor

Expression of Vascular Endothelial Growth Factor by Retinal Pigment Epithelial Cells Induced by Amyloid-� Is Depressed by an Endoplasmic Reticulum Stress Inhibitor

Expression of Vascular Endothelial Growth Factor by Retinal Pigment Epithelial Cells Induced by Amyloid-� Is Depressed by an Endoplasmic Reticulum Stress Inhibitor

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