Various substituted acetophenones (1-5) on treatment with iodine and thiourea yielded 2-amino-4-(substituted-phenyl)-thiazole (1a-5a), which on further treatment with various substituted aldehydes to get N-(substitutedbenzylidene)-4-(substitutedphenyl) thiazol-2-amine (1b-5b). All the synthesized compounds were characterized by their respective FTIR, 1H NMR and mass data. Synthesized compounds (1b-5b) were subjected to investigation for their antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus and Asperigillus fumigatus by disk diffusion method. Compound 5b was found to be most effective with largest zone of inhibition.Key words: thiazole, acetophenones, antibacterial, antifungal, substituted aldehydes

Fuloria, Neeraj Kumar

Fuloria, Shivkanya

Gupta, Rajul

INDONESIAN JOURNAL OF PHARMACY

English

Various substituted acetophenones (1-5) on treatment with iodine and thiourea yielded 2-amino-4-(substituted-phenyl)-thiazole (1a-5a), which on further treatment with various substituted aldehydes to get N-(substitutedbenzylidene)-4-(substitutedphenyl) thiazol-2-amine (1b-5b). All the synthesized compounds were characterized by their respective FTIR, 1H NMR and mass data. Synthesized compounds (1b-5b) were subjected to investigation for their antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus and Asperigillus fumigatus by disk diffusion method. Compound 5b was found to be most effective with largest zone of inhibition

Rajul Gupta

Neeraj Kumar Fuloria

Shivkanya Fuloria

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SYNTHESIS AND ANTIMICROBIAL ACTIVITY EVALUATION OF SOME SCHIFF BASES DERIVED FROM 2-AMINOTHIAZOLE DERIVATIVES

Indonesian Journal of Pharmacy

 Research Article Volume 24 Issue 3 (2013) 193 Indonesian J. Pharm. Vol. 24 No. 3 : 193 – 197 ISSN-p : 2338-9427 SYNTHESIS AND ANTIMICROBIAL ACTIVITY EVALUATION OF SOME SCHIFF BASES DERIVED FROM 2-AMINOTHIAZOLE DERIVATIVES  Rajul Gupta1*, Neeraj Kumar Fuloria2, Shivkanya Fuloria2  1Dept. Pharmacy, CMJ Univ., Modrina Mansion, Laitumkhrah, Shillong, Meghalaya-793 003, India 2Dept. Pharmaceutical Chemistry, Anuradha College of Pharmacy, Chikhli, Dist. Buldana, Maharashtra, India.  Submitted: 15-11-2012 Revised: 20-01-2013 Accepted: 16-03-2013  *Corresponding author Rajul Gupta  Email :  rajulgupta001@gmail.com ABSTRACT Various substituted acetophenones (1-5) on treatment with iodine and thiourea yielded 2-amino-4-(substituted-phenyl)-thiazole (1a-5a), which on further treatment with various substituted aldehydes to get N-(substitutedbenzylidene)-4-(substitutedphenyl) thiazol-2-amine (1b-5b). All the synthesized compounds were characterized by their respective FTIR, 1H NMR and mass data. Synthesized compounds (1b-5b) were subjected to investigation for their antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus and Asperigillus fumigatus by disk diffusion method. Compound 5b was found to be most effective with largest zone of inhibition.   Key words: thiazole, acetophenones, antibacterial, antifungal, substituted aldehydes.   INTRODUCTION Thiazole derivatives have attracted a great deal of interest owing to their anticancer activity (Kumar et al., 1993; Timita et al., 2002; Gorczynski et al., 2004), antibacterial activity (Karabasanagouda et al., 2008; Khan et al., 2012), antifungal activity (Karabasanagouda et al., 2008; Khan et al., 2012), anti-inflammatory activity (Karabasanagouda et al., 2008), anti-tubercular activity (Pattan et al., 2009), cardio-tonic activity (Giridhar et al., 2001), anti-degenerative activity on cartilage (Panico et al., 2003) etc. Thiazoles are known to be allosteric enhancer of A1 adenosine receptors (Goblyos et al., 2005) whereas other analogs are known to be inhibitors of protein phosphatases (Wipf et al., 2001). Heterocycle-bearing substrates are particularly desirable structures for screening and are prevalent in drugs that have reached the market place. The development of simple and general synthetic routes for widely used organic compounds from readily available reagents is one of the major challenges in organic chemistry. Therefore to meet the facile results of these tough challenges thiazole nucleus was  being considered. Among the wide variety of heterocycles that have been explored for developing pharmaceutically molecules, thiazole derivatives have played a vital role in the medicinal chemistry. There are large numbers of synthetic compounds with thiazole nucleus used for antimicrobial activities when properly substituted at 2-position.  In view of these observations and in continuation to develop better and potent antimicrobial agents, some newer thiazole derivatives were synthesized.   MATERIAL AND METHODS  Melting points were taken in open capillaries and are uncorrected. IR spectrum of compounds in KBr pellets were recorded on a FTIR-8400S spectrophotometer (SHIMADZU). 1HNMR spectra of the compounds were recorded on Bruker DRX 300 NMR spectrophotometer in DMSO-d6 using TMS as internal standard. Mass spectra of the compounds were recorded on MSN-9629          mass spectrometer. Elemental analysis was carried out on Elemental Vario EL III Carlo Erba 1108. The purity of compounds                 was  monitored  by  thin layer chromatography.  Synthesis and Antimicrobial Volume 24 Issue 3 (2013) 194 Thin layer chromatographic analysis of the compounds were performed on silica gel G coated glass plates using chloroform: methanol: pet. ether (9:1:0.5) as mobile phase. The spots were visualized by exposure to iodine vapours.  General method for the synthesis of (1a-5a)  Various substituted acetophenones (1-5) (0.01mol) were refluxed with iodine (0.01mol) and thiourea (0.02mol) for 9 hrs to get 2-amino-4-(substituted-phenyl)thiazole (1a-5a). The solid obtained was washed with diethyl ether, after which it was washed with sodium thiosulfate. Finally, it was washed with water and the residue was filtered, dried and recrystallized from distilled water.  General method for the synthesis of (1b-5b) 2-amino-4-(substituted-phenyl)-thiazole (1a-5b) (0.01mol) were refluxed with various substituted aromatic aldehydes (0.01mol) in ethanol along with glacial acetic acid (2-3 drops) for 5 hrs to get N-(substituted-benzylidene)-4-(substituted-phenyl)thiazol-2-amine (1b-5b). The final products were purified by recrystallization from water : DMF (1 : 1). N-(3-methoxybenzylidene)-4-phenyl-thiazol-2-amine (1b): Yield: 55.34 %; m.p. 107-108 ºC; Anal. Calcd. for C17H14N2OS: C, 69.36; H, 4.79; N, 9.52. Found: C, 69.33; H, 4.84; N, 9.47; IR (KBr, cm-1): 3062.28 (C-H stretching of aromatic ring), 2977.4 (=C-H stretching of N=C-H), 2945.76 (-C-H stretching of OCH3), 1674.86 (C=N stretching of N=C-H), 1586.24 (C-C stretching of aromatic ring), 1227.4 (C-O stretching of –OCH3), 674.38 (C-S stretching of thiazole); 1HNMR (DMSO-d6, δ / ppm): 3.317 (s, 3H, OCH3), 6.977 (s, 1H, =C-H), 7.087-7.875 (m, 9H, Aromatic), 8.187 (s, 1H, N=C-H). MS (m/z (relative abundance, %)): 294 (M+, 28.3), 264, 187 (BP, 100), 134, 133, 108, 104, 77.  N-(3-chlorobenzylidene)-4-phenyl-thiazol-2-amine (2b): Yield: 63.32 %; m.p. 117-118 ºC; Anal. Calcd. for C16H11ClN2S: C, 64.32; H, 3.71; N, 9.38. Found: C, 64.33; H,  Figure 1. Synthetic pathway  Table I. Derivation of the compound Where: Compound Number R1 R2  1b H m-methoxy  2b H m-chloro  3b H H  4b H p-methoxy  5b o-hydroxy p-chloro  Rajul Gupta Volume 24 Issue 3 (2013) 195 3.69; N, 9.39; IR (KBr, cm-1): 3064.77 (C-H stretching of aromatic ring), 2948.38 (=C-H stretching of N=C-H), 1678.56 (C=N stretching of N=C-H), 1569.38 (C-C stretching of aromatic ring), 776.58 (C-Cl stretching of m-chloro), 682.12 (C-S stretching of thiazole); 1HNMR (DMSO-d6, δ / ppm): 6.919 (s, 1H, =C-H), 7.057-7.896 (m, 9H, Aromatic), 8.189 ppm (s, 1H, N=C-H). MS (m/z (relative abundance, %)): 300 (M+2), 298 (M+, 31.6), 265, 187 (BP, 100), 138, 134, 133, 112, 77.  Synthesis of N-benzylidene-4-phenyl-thiazol-2-amine (3b): Yield: 58.58 %; m.p. 110-111 ºC; Anal. Calcd. for C16H12N2S: C, 72.70; H, 4.58; N, 10.60. Found: C, 72.72; H, 4.56; N, 10.62; IR (KBr, cm-1): 3053.11 (C-H stretching of aromatic ring), 2961.88 (C-H stretching of N=C-H), 1623.95 (C=N stretching of N=C-H), 1525.59 (C-C stretching of aromatic ring), 628.86 (C-S stretching of thiazole); 1HNMR (DMSO-d6, δ / ppm): 6.588 (s, 1H, =C-H), 7.195-7.394 (m, 10H, Aromatic), 8.188 ppm (s, 1H, N=C-H); MS (m/z (relative abundance, %)): 264 (M+, 37.4), 187 (BP, 100), 134, 133, 104, 77.  Synthesis of N-(4-methoxybenzyli-dene)-4-phenylthiazol-2-amine (4b): Yield: 66.41 %; m.p. 111-112 ºC; Anal. Calcd. for C17H14N2OS: C, 69.36; H, 4.82; N, 9.52. Found: C, 69.34; H, 4.81; N, 9.51; IR (KBr, cm-1): 3060.82 (C-H stretching of aromatic ring), 2977.89 (C-H stretching of N=C-H), 2906.53 (C-H stretching of OCH3), 1662.52 (C=N stretching of N=C-H), 1581.52 (C-C stretching of aromatic ring), 1240.14 (C-O stretching of OCH3), 653.82 cm-1 (C-S stretching of thiazole); 1HNMR (DMSO-d6, δ / ppm): 3.311 (s, 3H, OCH3), 6.907 (s, 1H, =C-H), 7.084-7.825 (m, 9H, Aromatic), 8.188 ppm (s, 1H, N=C-H). MS (m/z (relative abundance, %)): 294 (M+, 28.3), 264, 187 (BP, 100), 134, 133, 108, 104, 77.  Synthesis of 2-(2-(4-Chlorobenzyli-deneamino)thiazol-4-yl)phenol (5b): Yield: 67.68 %; m.p. 125-126 ºC; Anal. Calcd. for C16H11ClN2OS: C, 61.05; H, 3.52; N, 8.90. Found: C, 61.01; H, 3.55; N, 8.89; IR (KBr, cm-1): 3060.82 (C-H stretching of aromatic ring), 2977.89 (C-H stretching of N=C-H), 2906.53 (C-H stretching of OCH3), 1662.52 (C=N stretching of N=C-H), 1581.52 (C-C stretching of aromatic ring), 1240.14 (C-O stretching of C-OH), 770.45 (C-Cl stretching of p-chloro), 653.82 cm-1 (C-S stretching of thiazole); 1HNMR (DMSO-d6, δ / ppm): 4.480 (s, 1H, OH), 6.103 (s, 1H, =C-H), 6.327-7.777 (m, 8H, Aromatic), 8.458 ppm (s, 1H, N=C-H). MS (m/z (relative abundance, %)): 316 (M+2), 314 (M+,21.4),203 (BP,100), 150, 149, 138, 112, 77.   Antimicrobial activity The synthesized compounds 1-5 were screened for antibacterial (S. aureus, E. coli, P. aeruginosa) and antifungal (C. albicans, A. flavus, A. fumigatus) activities by disk diffusion method at a concentration of 2mg/mL using DMF as a solvent. The results were recorded in duplicate using ciprofloxacin and fluconazole as standards and are given in table II and III.  RESULTS AND DISCUSSION Various substituted acetophenones         (1-5) reacted with iodine and thiourea                to get 2-Amino-4-(substituted-phenyl)-thiazole  (Sutariya et al., 2007) (1a-5a). Next, 2-amino-4-(substituted-phenyl)-thiazole (1a-5a) reacted with various substituted aldehydes to get N-(substitutedbenzylidene)-4-(substitutedphenyl) thiazol-2-amine (1b-5b). In this reaction, amino group of thiazole (1a-5a) was treated with various aromatic aldehydes to get corresponding Schiff's bases (1b-5b).The FTIR spectra of compounds 1b-5b exhibited bands in the region of 2948.38-2977.89cm-1 due to C-H stretching of N=C-H. In 1H NMR spectra of compounds 1b-4b, one proton singlet appeared between δ 8.187-8.458ppm was assigned to N=C-H proton. MS of representative compounds 1b-5b exhibited molecular ion peak at m/z. The structures of the synthesized compounds were assigned on the basis of elemental analysis, 1H NMR, FTIR and Mass spectral data and physical data. The synthesized compounds were screened for antibacterial and antifungal activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus and Asperigillus fumigatus by disk diffusion method. In figure 1, compound 5b was found to be most effective with the largest zone of inhibition against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus      and Asperigillus fumigates. Compound 2b was  also  found  to  be  very  effective against all the Synthesis and Antimicrobial Volume 24 Issue 3 (2013) 196 microorganisms after compound 5b. Compound 1b and 4b were also reported with the significant activities against the tested microorganisms.   CONCLUSION Both analytical and spectral data (IR, 1H-NMR, MS) of all the synthesized compounds were in full agreement with the proposed structure. After comparing the antimicrobial results of compounds 5a-5b, it was concluded that compound 5b was found to possess maximum activity against tested strains. This maximum activity may be due to presence of both hydroxy and chloro group in compound 5b.  ACKNOWLEDGEMENT The authors are thankful to IIT, Delhi and CDRI, Lucknow for providing facilities.  REFERENCES Giridhar, T., Reddy, RB., 2001, Aminothiazoles: Part1- Syntheses & Pharmacological evaluation of 4-[isobutylphenyl]-2-substituted-aminothiazoles. Indian J Chem., 40B, 1279-1281 Goblyos, A., Santiago, SN., Pietra, D., Krieger, TM., Kunzel, JV., Brussee, J., Ijzerman, AP., 2005, Synthesis and biological evaluation of 2-aminothiazoles and their amide derivatives on human adenosine receptors. Lack of effect of 2-aminothiazoles as allosteric enhancers. Bioorg. Med. Chem., 13, 2079-2087. Gorczynski, MJ., Leal, RM., Mooberry, SL., Bushweller, JH., Brown, ML., 2004, Synthesis and evaluation of substituted 4-aryloxy- and 4-arylsulfanyl-phenyl-2-aminothiazoles as inhibitors of human breast cancer cell proliferation. Bioorg. Med. Chem., 12, 1029-1036   Karabasanagouda, T., Adhikari, AV., Dhanwad, R., Parameshwarappa, G., 2008, Synthesis of some new 2-(4-alkylthiophenoxy)-4-substituted-1, 3-thiazoles as possible anti-inflammatory Table II. Antibacterial activity of compounds (1b-5b)  Zone of Inhibition (mm) Compounds S. aureus E. coli P. aeruginosa 1b.  17.3 ± 0.33 16.3 ± 0.00 17 ± 0.00 2b.  19.2 ± 0.00 20.2 ± 0.00 20.3 ± 0.33 3b.  16.4 ± 0.00 15 ± 0.00 15.5 ± 0.00 4b.  17.3 ± 0.00 17 ± 0.33 17 ± 0.67 5b.  21.5 ± 0.00 20.5 ± 0.00 20.4 ± 0.00 Ciprofloxacin 27 ± 0.00 28 ± 0.00 27 ± 0.00 DMF - - -  All the values are expressed as mean ± SEM of triplicates  Table III. Antifungal activity of compounds (1b-5b)  Zone of Inhibition (mm) Compounds C. albicans A. flavus A. fumigatus 1b.  10.3 ± 0.33 11 ± 0.00 10 ± 0.00 2b.  13.4 ± 0.00 13 ± 0.00 12.2 ± 0.00 3b.  10.3 ± 0.33 9.3 ± 0.33 9.3 ± 0.33 4b.  9.3 ± 0.33 10.7 ± 0.67 11 ± 0.00 5b.  13.5 ± 0.00 14.0 ± 0.00 13.5 ± 0.00 Fluconazole 17 ± 0.00 16 ± 0.00 17 ± 0.00 DMF - - -  All the values are expressed as mean ± SEM of triplicates  Rajul Gupta Volume 24 Issue 3 (2013) 197 and antimicrobial agents. Indian J. Chem., 47B, 144-152 Khan, KM., Ambreen, N., Karim, A., Saied, S., Amyn, A., Ahmed, A., Perveen, S., 2012, Schiff Bases of Thiazole as Antibacterial and Antifungal Agents. J. Pharmacy Res., 5, 651-656 Kumar, Y., Green, R., Wise, DS., Wotring, L. L., Townsend, LB., 1993, Synthesis of 2,4-disubstituted thiazoles and selenazoles as potential antifilarial and antitumor agents. 2. 2-Arylamido and 2-alkylamido derivatives of 2-amino-4-(isothiocyanatomethyl)thiazole and 2-amino-4-(isothiocyanatomethyl) selenazole. J. Med. Chem., 36, 3849-3852 Panico, AM., Geronikaki, A., Mgonzo. R., Cardile, V., Gentile, B., Doytchinova, I., 2003, Aminothiazole derivatives with antidegenerative activity on cartilage. Bioorg. Med. Chem., 11, 2983-2989 Pattan, SR., Bukitagar, A. A., Kapadnis, B. P., Jadhav, S. G., 2009, Synthesis and evaluation of some new substituted phenylthiazole derivatives and their antitubercular activity. Indian J. Chem., 48B, 1033-1037 Sutariya, B., Raziya, SK., Mohan, S., Sambasiva, SV., 2007, Synthesis and antimicrobial activity of some new 2-substituted aminothiazoles. Indian J. Chem., 46B, 884-887 Tomita, K., Tsuzuki, Y., Shibamori, K., Tashima, M., Kajikawa, F., Sato, Y., Kashimoto, S., Chiba, K., Hino, K., 2002, Synthesis and structure-activity relationships of novel 7-substituted 1,4-dihydro-4-oxo-1-(2-thiazolyl)-1,8-naphthyridine-3-carboxylic acids as antitumor agents. Part 1. J. Med. Chem., 45, 5564-5575 Wipf, P., Aslan, DC., Southwick, EC., Lazo, J. S., 2001, Sulfonylated aminothiazoles as new small molecule inhibitors of protein phosphatases. Bioorg. Med. Chem. Lett., 11, 313-317  

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SYNTHESIS AND ANTIMICROBIAL ACTIVITY EVALUATION OF SOME SCHIFF BASES DERIVED FROM 2-AMINOTHIAZOLE DERIVATIVES

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