The estimation of GHG emission from domestic livestock in the Republic of Macedonia during the period from 2007-2012 is performed according to the IPCC Good Practice Guidance and Uncertainty Management in National Greenhouse Gas Inventories applying the Tier 1 method for calculation of emissions. This article has accepted the following gasses: Methane (CH4), Nitrous oxide and CO2-eq from the domestic livestock sub sector using data sources from the official Statistical Yearbooks of the Republic of Macedonia. According to available data for livestock species, distribution of total annual methane emission and nitrogen excretion was calculated in each planning region for the period 2007-2012. In the analyzed period two regions showed continuously highest (Southeast region) and lowest (Vardar region) CH4 emission and nitrogen excretion. In the period 2007-2012 CH4 emission ranged between 26.43 Gg (2007) and 23.78 Gg (2012), with the lowest value in 2012 (23.78 Gg). Depending on the animal waste management system the highest value for nitrogen excretion (solid storage and drylot) was observed in 2010 (kt/N/yr). Almost similar value for nitrogen excretion from pasture and paddock was observed in 2007 and 2008, 9.54 and 9.53 kt/N/yr respectively, with the lowest value for analyzed period noted in 2009 (8.82 kt/N/yr). In this period the highest value (3.32 kt/N/yr) for other type of AWMS was recorded in 2007. Separation of certain regions in the country regarding GHG emission can be clearly noted. Data for the period 2007-2012 show a downward trend in GHG emission. Applications of modern breeding technology, balanced feed as well as better feed quality in the future are the main objectives in order to reduce GHGs emission from this subsector