Furijeova transformacija - spektroskopija u bliskoj infracrvenoj oblasti za brzo i nedestruktivno merenje sadržaja amiloze u zrnu pirinča

Abstract

The quality and quantity of amylose are two important factors that determine the quality of paddy. The feasibility of developing a technique for rapid monitoring of paddy quality by using Fourier Transform - Near Infrared (FT-NIR) Spectroscopy combined with chemometrics was investigated. Spectra of 250 paddy samples were collected by using an integrating sphere accessory. The amylose content was analyzed by reference method. It ranged from 13.24 to 27.93%. The spectral data was analyzed by partial least squares (PLS) algorithm and calibration model generated. Correlation coefficient (R2) for the calibration model was >0.78 with root mean square error of estimation (RMSEE) 0.72 and root mean square error of cross-validation (RMSECV) 0.78 sa srednjim kvadratom odstupanja 0.72 i srednji kvadrat odstupanja unakrsne procene < 2. FT-NIR spektroskopijom su identifikovani uzorci koji sadrže amilozu sa talasnim brojevima 5184 i 6834 cm-1. Tako je razvijen brz, jednostavan i pouzdan metod određivanja sadržaja amiloze