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Ciofani, Gianni

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Engineering Conferences InternationalECI Digital ArchivesNanotechnology in Medicine: From Molecules toHumans Proceedings7-6-2016Active nanomaterials for biomedical applicationsGianni CiofaniItalian Institute of Technology, Italy, gianni.ciofani@polito.itFollow this and additional works at: http://dc.engconfintl.org/nanotech_medPart of the Engineering CommonsThis Abstract and Presentation is brought to you for free and open access by the Proceedings at ECI Digital Archives. It has been accepted for inclusionin Nanotechnology in Medicine: From Molecules to Humans by an authorized administrator of ECI Digital Archives. For more information, pleasecontact franco@bepress.com.Recommended CitationGianni Ciofani, "Active nanomaterials for biomedical applications" in "Nanotechnology in Medicine: From Molecules to Humans",Prof. Lola Eniola-Adefeso, Department of Chemical Engineering, University of Michigan, USA Prof. Paolo Decuzzi, Italian Institute ofTechnology, Italy Eds, ECI Symposium Series, (2016). http://dc.engconfintl.org/nanotech_med/17Active nanomaterials for biomedical applicationsPolytechnic University of TorinoDIMEASCorso Duca degli Abruzzi, 2410129 Torino, Italygianni.ciofani@polito.itGianni CiofaniItalian Institute of TechnologyCenter for Micro-BioRobotics @SSSAViale Rinaldo Piaggio, 3456025 Pontedera (Pisa), Italygianni.ciofani@iit.itNanotechnology in Medicine:From Molecules to HumansJuly 3-7, 2016 Hernstein, AustriaOUR GROUPPolitecnico di TorinoIstituto Italiano di TecnologiaPIEZOELECTRICITY IN NANOMEDICINEPolitecnico di TorinoIstituto Italiano di TecnologiaACS Nano, 9(7): 7678-7689 (2015)ACS Nano, 4(10): 6267–6277 (2010)Piezoelectric nanomaterialsCiofani G., Genchi G.G. and Mattoli V. ZnO nanowirearrays as substrates for cell proliferation and differentiation. Mat Sci Eng C 32: 341-347 (2012)ZnO nanorodsBoron nitridenanotubesBarium titanatenanoparticlesRocca A., […], Ciofani G. Barium titanatenanoparticles and hypergravity stimulationimprove differentiation of mesenchymal stem cells into osteoblasts. Int. J. Nanomed. 10: 433-445 (2015) Ciofani G., […], Mattoli V. Cytocompatibility evaluation of gum Arabic-coated ultra-pure boron nitride nanotubes on human cells. Nanomed. UK 9: 773-788 (2014) Politecnico di TorinoIstituto Italiano di TecnologiaBarium Titanate Nanoparticles20 µm2θIntensity(a.u.)10 20 30 40 50 60 70 80020040060080010001200140016001800G. Ciofani, […], V. Mattoli, Effects of barium titanate nanoparticles on proliferation and differentiation of mesenchymal stem cells. Coll. Surf. B: Biointerfaces 102: 312-320 (2013)Intensity(a.u.)1004006008001000120014002002θ20 30 40 50 60 70 80TetragonalCubicex: 633 nmem: 645-745 nmPolitecnico di TorinoIstituto Italiano di TecnologiaBarium Titanate NanoparticlesPolitecnico di TorinoIstituto Italiano di TecnologiaBTNP-MEDIATED STIMULATION0 20 40 60 80 100 120 140 160 180-0.50.00.51.01.52.0time (s)ΔF/F0ΔF/F0US (0.8 W/cm2) + BTNPsΔF/F02.00.00 20 40 60 80 100 120 140 160 180-0.50.00.51.01.52.0time (s)ΔF/F0ΔF/F0US (0.8 W/cm2)ΔF/F02.00.0Marino A., […], Ciofani G. Piezoelectric nanoparticle-assisted wireless neuronal stimulation. ACS Nano, 9(7): 7678-7689 (2015)Politecnico di TorinoIstituto Italiano di Tecnologia0 20 40 60 80 100 120 140 160 180-0.40.00.40.81.21.62.0time (s)?F/F0?F/F0US (0.8 W/cm2) + cubic crystal BTNPs?F/F02.00.0P(VDF-TrFE)/BaTiO3 nanoparticle composite films Politecnico di TorinoIstituto Italiano di TecnologiaGenchi G.G. […], Ciofani G. P(VDF-TrFE)/BaTiO3 nanoparticle composite films mediate piezoelectric stimulation and promote differentiation of SH-SY5Y neuroblastoma cells. Advanced Healthcare Materials, 10.1002/adhm.201600245Sample preparation and characterization: Electron Microscopy10 µm10 µmP(VDF-TrFE)P(VDF-TrFE) + BTNPsP(VDF-TrFE) + BTNPs (cryosection)1) Powder dissolution/dispersion in   methylethylketone:200 mg/ml P(VDF-TrFE, 70/30)40% P(VDF-TrFE) and 60% BTNPs (w/w)2) Sonication at 8 W for 10 min3) Casting4) Annealing at 40°C5) Under vacuum for 12 h10 µmPolitecnico di TorinoIstituto Italiano di TecnologiaAFMPFMP(VDF-TrFE) + BTNPsP(VDF-TrFE)Politecnico di TorinoIstituto Italiano di TecnologiaSample preparation and characterization: AFM & PFMX-Ray Diffraction52.632.3β phase68.459.4total15.827.1α phaseCrystallinity(%)P(VDF-TrFE) + BTNPsP(VDF-TrFE)X-ray diffractionPolitecnico di TorinoIstituto Italiano di Tecnologia0501001502002503000.002.004.006.008.0010.0002468101214020040060080010001200P(VDF-TrFE)/ BTNPsP(VDF-TrFE) P(VDF-TrFE)/ BTNPsUltimate tensile strength (MPa)P(VDF-TrFE)Young’s modulus (MPa)Extension at maximum load (%)P(VDF-TrFE)Extension at break (%)P(VDF-TrFE)***P(VDF-TrFE)/ BTNPsP(VDF-TrFE)/ BTNPsEXTENSIMETRYCube-within-cube model by Ogorkiewicz and WeidmannPolitecnico di TorinoIstituto Italiano di Tecnologia0246810120 20 40 60 80 100 120 140 160 180Tensile stress (MPa)Tensile strain (%)P(VDF-TrFE)P(VDF-TrFE)/BTNPs0246810120 5 10 15 20Piezoresponse characterization0.240.110.059g31,1[mV/N]53.511.81.1d31,1[pm/V]23.711.122.03εS25.7122.1εr22.518.622.7Q15.756.14.0Vout1 [mVRMS]P(VDF-TrFE) + BTNPsP(VDF-TrFE)PTFE (non piezo)res./samplePolitecnico di TorinoIstituto Italiano di TecnologiaCa2+ imaging0 10 20 30 40 50 60 70-0.51.53.55.57.5Time (s)∆F/F0CtrlUS0 10 20 30 40 50 60 70-0.51.53.55.57.5∆F/F0P(VDF-TrFE)USTime (s)0 10 20 30 40 50 60 70-0.51.53.55.57.5P(VDF-TrFE) + BTNPsUS∆F/F0Time (s)Acute ultrasound stimulation:1 W/cm2, 100 Hz Burst RatePolitecnico di TorinoIstituto Italiano di TecnologiaNeurite emission and analysis50 µmCtrlUS-P(VDF-TrFE)P(VDF-TrFE) +BTNPsUS+TubulinNuclei020406080100120140US- US+Ctrl P(VDF-TrFE) P(VDF-TrFE) + BTNPsNeurite length (µm)US- US+ US- US+Chronic ultrasound stimulation: 1 W/cm2, 100 Hz Burst Rate, 2X/day, 6 dd*p<0.01***Politecnico di TorinoIstituto Italiano di TecnologiaDIRECT LASER WRITING• 3D Laser Lithography• Resolution < 100 nm• Two-photon polymerization (laser 780 nm, power > 60 mW)• Dedicated resistPolitecnico di TorinoIstituto Italiano di TecnologiaDIRECT LASER WRITINGPolitecnico di TorinoIstituto Italiano di TecnologiaDIRECT LASER WRITING: THE PROCESSPolitecnico di TorinoIstituto Italiano di TecnologiaTHE OSTEOPRINT50 µm3D model of trabecular bone Adhesion of SaOS-2 cellsDifferentiation of SaOS-2 cells (confocal & SEM)Marino A., […], Ciofani G. The Osteoprint: A two-photon polymerized 3D structure for the enhancement of bone-like cell differentiation. ActaBiomaterialia, 10(10): 4303-4313 (2014)Politecnico di TorinoIstituto Italiano di TecnologiaBTNP-DOPED RESIST• BTNP mixed with ormocomp• Sonication• 2pp process• SEM/FIB/EDX analysis: BTNP uniformely dispersed in the structuresPolitecnico di TorinoIstituto Italiano di TecnologiaBTNP-DOPED STRUCTURES• 2pp of complex structures• BTNP uniformely dispersed in the 3D scaffolds• SHG signal detectionPolitecnico di TorinoIstituto Italiano di TecnologiaBTNP-DOPED STRUCTURES10864201086420X[µm]Y[µm] 68.84 nm 0.00 nm10864201086420X[µm]Y[µm] 899.34 mV 42.72 mVPiezoelectric characterization: AFM & PFMPiezoresponse amplitudeTopographyd33 = 0.6 pm/VPolitecnico di TorinoIstituto Italiano di TecnologiaBTNP-DOPED “OSTEOPRINTS”BTNPs actin nuclei mergingPolitecnico di TorinoIstituto Italiano di TecnologiaMarino A., […], Ciofani G. Two-photon lithography of 3D nanocomposite piezoelectric scaffolds for cell stimulation. ACS Applied Materials and Interfaces, 7(46): 25574-25579 (2015)PIEZOELECTRIC STIMULATIONOsteoimageOP OP + US OP + BTNPs OP + US + BTNPs• Human Saos-2 cell testing• Stimulation with US for 5 days (30 s, 3 times per day, 0.8 W/cm2)HA depositarea (%)OP OP + US OP + BTNPs OP + BTNPs + US0**246810121416• Evaluation of the mineralization• Osteoimage staining• Quantification of HA depositsPolitecnico di TorinoIstituto Italiano di TecnologiaCONCLUSIONS• 2D/3D micro/nanofabrication• High resolution• Nanostructured surfaces• Biomimetic substrates• Tuning of physicalpropertiesMarino A., […], Ciofani G. Biomimicry at the nanoscale: Current research and perspectives of two-photon polymerization. Nanoscale, 7(7): 2841–2850 (2015)Politecnico di TorinoIstituto Italiano di TecnologiaThank you for your attention!Politecnico di TorinoIstituto Italiano di Tecnologia

Active nanomaterials for biomedical applications

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Active nanomaterials for biomedical applications

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