Christakopoulos , Paul

Xiros , Charilaos

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Engineering Conferences International

Bioenergy I Posters: Fuel AlcoholsECI -Engineering Conferences InternationalDirect bioconversion of brewers’ spent grain to ethanolAuthors: Charilaos Xiros and Paul ChristakopoulosAddress: Biotechnology LaboratoryDepartment of Product and Process DevelopmentSchool of Chemical EngineeringNational Technical University of AthensIroon Polytechniou 5, 15780 ATHENS, GREECEchaxiros@ntua.central.ntua.grBrewers’ spent grain (BG) is a low-value co-product of the brewing process. BG is rich in cellulose (17%) and non-cellulolosic polysaccharides (mainly arabinoxylans) (39%) Fusarium oxysporum have been reported to acquire the ability of fermenting cellulose and hemicellulose directly to ethanolF. oxysporum is used as the fermentation organism, it is not necessary to perform a separate enzymic hydrolysis of the lignocellulosic raw material, as this microorganism can produce the necessary enzymes The production of  cellulose and hemicellulose degrading enzymes was studied in several conditionsEnzyme Microbial Technology, 11, 236-239, 1989Enzyme Microbial Technology, 36, 693-699, 2005Ethanol production from brewers’ spent grain by F. oxysporumBioreactorAerobic growthBioreactorAnaerobic growthHemi-cellulasesCelluloseHemic/lose+MicroorganismCellulases Microorganism(CellulasesHemicellulases)CelluloseHemic/loseGlucosePentoseMicroorganismETHANOLLignocellulosicmaterialsi ll l it ri l Pretreatment Ethanol fermentationStudied factors affecting bioethanol production • Production of cellulolytic and hemicellulolytic enzymes• pH • Aeration level• A mixed substrate (corn cob - spent grain) and four days of aerobic growth found to be the optimum for the enzyme production• Achieved activities  for the degrading enzymes:The mixed substrate in the ratio 1:1 was found to give the optimum activities: 133.3 U/mL for xylanase,  22.47 U/mLfor endoglucanase, 0.47 U/mL for β-glucosidase, 0.064 U/mL for xylosidase, 0.159 U/mL for arabinofuranosidase, 1.03 U/mL for exoglucanase and 0.014 U/mL for feruloylesterase• Ethanol production was carried out in 6% of spent grain

POSTER: Direct Bioconversion of Brewers’ Spent Grain to Ethanol

https://dc.engconfintl.org/cgi/viewcontent.cgi?article=1041&amp;context=bioenergy_i

POSTER: Direct Bioconversion of Brewers’ Spent Grain to Ethanol

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Engineering Conferences International