Liao, Bo

English

Engineering Conferences International

Use of Genetically Modified Saccharomycescerevisiae to Convert Starch Directly to BioethanolBo Liao, G.A. Hill* and W.J. RoeslerDepartment of Biochemistry*Department of Chemical EngineeringUniversity of SaskatchewanIndustrial fermentation processCookingGrindingRaw StarchFermentationLiquefactionEnzymesSaccharificationEnzymesYeastPossible Solution-Strategy IEthanolPossible Solution-Strategy IIEthanolStructure of Yeast expression plasmidYeast expression plasmidStructure of the fusion genespAnchoredpSecretedStarch plate assayW.T. pAnchored pSecretedBatch fermentation on starch  Batch fermentation on starch  (cont.)Batch fermentation on starch with 0.5% w/v glucoseBatch fermentation on starch with 0.5% w/v glucose (cont.)Batch fermentation on starch with 0.5% w/v glucose (cont.)2.533.54202530Ethanol (g/L)Starch concentration (g/L)Starch(W.T.) Starch(pSecreted)Ethanol(W.T.) Ethanol(pSecreted)00.511.520510150 20 40 60 80 100 120Ethanol (g/L)Starch concentration (g/L)Time (hours)Batch fermentation on starch with 0.5% w/v glucose and 5 U/L of glucoamylaseBatch fermentation on starch with 0.5% w/v glucose and 5 U/L of glucoamylase (cont.)Batch fermentation on starch with 0.5% w/v glucose and 5 U/L of glucoamylase (cont.)2.533.54202530Ethanol (g/L)Starch concentration (g/L)Starch(pSecreted) Starch(pSecreted+GM)Ethanol(pSecreted) Ethanol(pSecreted+GM)00.511.520510150 20 40 60 80 100 120Ethanol (g/L)Starch concentration (g/L)Time (hours)Batch fermentation on starch with 0.5% w/v glucose and 5 U/L of glucoamylase (cont.)2.533.54202530Ethanol (g/L)Starch concentration (g/L)Starch(W.T.+GM) Starch(pSecreted+GM)Ethanol(W.T.+GM) Ethanol(pSecreted+GM)00.511.520510150 20 40 60 80 100 120Ethanol (g/L)Starch concentration (g/L)Time (hours)SummarySummary (cont.)VS.pSecreted pAnchoredFuture worksYeast integration plasmid-stability-high level expressionAcknowledgement Dr. William Roesler Dr. Gordon Hill Ashley FultonAgriculture Development Fund (ADF)Agriculture Canada’s ABIP FundSolar eclipseQuestions?W.T. pAnchored pSecreted in 1999Breaking down the starch molecules α-AmylaseGlucoamylase

Use of Genetically Modified Saccharomyces Cerevisiae to Convert Starch Directly to Bioethanol

https://dc.engconfintl.org/cgi/viewcontent.cgi?article=1025&amp;context=co2_summit

Use of Genetically Modified Saccharomyces Cerevisiae to Convert Starch Directly to Bioethanol

Abstract

Similar works

Full text

Available Versions

Engineering Conferences International