Berruti, Franco

Kersten, Sascha

Luque, Luis

Rehmann, Lars

van Rossum, Guus

Westerhof, Roel

English

Engineering Conferences International

Engineering Conferences InternationalECI Digital ArchivesBioEnergy IV: Innovations in Biomass Conversionfor Heat, Power, Fuels and Chemicals ProceedingsSpring 6-13-2013Alcohol production from pyrolytic sugars obtainedfrom selective fast pyrolysis of pretreated woodLuis LuqueWestern UniversityFranco BerrutiWestern UniversitySascha KerstenWestern UniversityGuus van RossumUniversity of TwenteRoel WesterhofICFARSee next page for additional authorsFollow this and additional works at: http://dc.engconfintl.org/bioenergy_ivPart of the Chemical Engineering CommonsThis Conference Proceeding is brought to you for free and open access by the Proceedings at ECI Digital Archives. It has been accepted for inclusion inBioEnergy IV: Innovations in Biomass Conversion for Heat, Power, Fuels and Chemicals by an authorized administrator of ECI Digital Archives. Formore information, please contact franco@bepress.com.Recommended CitationLuis Luque, Franco Berruti, Sascha Kersten, Guus van Rossum, Roel Westerhof, and Lars Rehmann, "Alcohol production frompyrolytic sugars obtained from selective fast pyrolysis of pretreated wood" in "BioEnergy IV: Innovations in Biomass Conversion forHeat, Power, Fuels and Chemicals", Manuel Garcia-Perez,Washington State University, USA Dietrich Meier, Thünen Institute of WoodResearch, Germany Raffaella Ocone, Heriot-Watt University, United Kingdom Paul de Wild, Biomass & Energy Efficiency, ECN, TheNetherlands Eds, ECI Symposium Series, (2013). http://dc.engconfintl.org/bioenergy_iv/40AuthorsLuis Luque, Franco Berruti, Sascha Kersten, Guus van Rossum, Roel Westerhof, and Lars RehmannThis conference proceeding is available at ECI Digital Archives: http://dc.engconfintl.org/bioenergy_iv/40ALCOHOL PRODUCTION FROM PYROLYTIC SUGARS OBTAINED FROM SELECTIVE FAST PYROLYSIS OF PRETREATED WOODVAN ROSSUM, G.,  BERRUTI, F.,  KERSTEN, S., WESTERHOF, R.,  REHMANN, L.LUIS CARLOS LUQUE-MORENOPHD CANDIDATEWESTERN UNIVERSITYJUNE 13, 2013BIOENERGY IV Background Previous studies showed that feedstock demineralization increases levoglucosan (LG) yield (Oudenhoven, 2012) LG is the most abundant anhydrous sugar in demineralized pyrolytic oil  If acid hydrolyzed it is transformed to glucose Figure 1. Levoglucosan hydrolysis to glucose Present among a pool of fermentation inhibitors  HMF Phenolics: Lignin derived compounds 2H2SO4ΔBackground Detoxification with Activated carbon  Polymers  XAD-4 XAD-7  More steps that are avoidable 3Objective Production of a fermentable substrate from demineralized (acid washed) biomass pyrolytic oil.   Developing a high throughput analytical metodolody for pyrolysis oil fermentabilityassesment4Demineralization (acid wash)• Removing ion• Increases LG yield• Decreases water contentMethodology 5Cold Water extraction• Precipitates insoluble ligninEthyl acetate extraction• Removes soluble growth inhibitory compounds Fermentation• Transforms pyrolytic sugar into ethanolHydrolysis & Neutralization• Precipitates any left acidic compoundsResults 68.25 1.05 3.91 0.06 0.19Molar Yield 0.43 Molar Yield 0.497.15 0 0.36 0.00Neutralized hydrolyzate 14.78 2.75 3.8 0.08 0.10Glucose (g/L)Levoglucosan carbon fraction Glucose carbon fraction Bio-oil extract 17.225 7.9 0 0.20 0.00 8.9NON ACID WASHED BIO-OIL Cold Water Extraction Ethyl Acetate Extraction TOC       (g/L)Levoglucosan   (g /L)Glucose (g/L)Levoglucosan carbon fraction Glucose carbon fraction TOC       (g/L)Levoglucosan   (g /L)36.7 1.32 43.4 0.02 0.47Molar Yield 0.84 Molar Yield 0.8844.5 0 0.48 0.00Neutralized hydrolyzate 38.5 1 41.8 0.01 0.43Glucose (g/L)Levoglucosan carbon fraction Glucose carbon fraction Bio-oil extract 46.9 44.6 0.8 0.42 0.00 41.3ACID WASHED BIO-OIL Cold Water Extraction Ethyl Acetate Extraction TOC       (g/L)Levoglucosan   (g /L)Glucose (g/L)Levoglucosan carbon fraction Glucose carbon fraction TOC       (g/L)Levoglucosan   (g /L)Table 1. Non-Acid washed bio oil extractions Table 2. Acid washed bio oil extractions Results 7After ethyl acetateextractionAfter waterextractionFigure 1.  Fermentability test of the extracts obtained from acid washed bio-oil• Water extraction is not sufficient to extract the soluble inhibitory compounds• Further ethyl acetate extraction helps to increase cell densityResults 8Figure 2. Lag time and specific growth rate in pyrolysis extract hydrolyzate fermentationFermentation Parameters• Specific growth rate decreases with increasing pyrolytic sugar content.• Lag time decreased in the ethyl acetate extracted fermentationAfter ethyl acetateextractionAfter waterextractionResults 9Ethyl acetate extractedfermentation productsWater extract fermentationproductsFigure  3.  Glucose and ethanol concentration after fermentation is completed. Ethanol yield showed in red• Increasing the pyrolytic sugar content impacts heavily cell growth hence the productivity.• When water extract is fermented, only 20%of it can be co-feeded with fresh YPG media.Conclusion  Cell density is not directly correlated with ethanol yield Neutralization of the hydrolyzate helps removing inhibitory compounds  Removing left over soluble phenolics via ethylacetate extraction increases the fermentability of the extract to 100% Assay indicates to be a potential process to assessPyrolysis oil fermentability Scale up Moving towards other fuels production, likebutanol10Acknowledgements 11Thank you for listening! 12Questions?Contact informationLuis Carlos Luque-MorenoDepartment of Chemical and Biochemical EngineeringICFARWestern UniversityLondon, Ontario  lluque@uwo.caReferences 131Briens, C., J. Piskorz & F. Berruti (2008) Biomass valorization for fuel and chemicals production - A review. International Journal of Chemical Reactor Engineering2Kersten, S., Garcia-Perez, M (2013). Recent developments in fast pyrolysis of ligno-cellulosic materials. Current Opinion in Biotechnology. Article in press3Li, L., Zhang, H.X. 2004. Preparing levoglucosan derived from waste material by pyrolysis. Energy Sources, 26(11), 1053-1059.4Lian, J.N., Chen, S.L., Zhou, S.A., Wang, Z.H., O'Fallon, J., Li, C.Z., Garcia-Perez, M. (2010). Separation, hydrolysis and fermentation of pyrolytic sugars to produce ethanol and lipids. Bioresource Technology, 101(24), 9688-9699. 5Lin, S.-H., Juang, R.-S. (2009). Adsorption of phenol and its derivatives from water using synthetic resins and low-cost natural adsorbents: A review. Journal of Environmental Management, 90(3), 1336-1349.6 Oudenhoven, S.R.G., Westerhof, R.J.M., Aldenkamp, N., Brilman, D.W.F., Kersten, S.R.A. (2012)Demineralization of wood-derived acid: Towards a selective pyrolysis process for fuel and chemicals production. Journal of Analytical and Applied Pyrolysis. Article in press

Alcohol production from pyrolytic sugars obtained from selective fast pyrolysis of pretreated wood

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Alcohol production from pyrolytic sugars obtained from selective fast pyrolysis of pretreated wood

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