Shi , Zhongping

Sun , Zhihao

English

Engineering Conferences International

SYTU 1Zhihao Sun & Zhongping Shi The acetone-butanol (ABE) fermentation industries in ChinaThe Key Laboratory of Industrial Biotechnology, Ministry of EducationSchool of Biotechnology, Southern Yangtze University (SYTU)Wuxi 214036, China    i l i l , i i   il  i l ,   i i  i , iSYTU 2Scopes of The Presentation?History of Acetone-Butanol Fermentative Industries in China?Acetone-Butanol Industrial Fermentation Techniques in China?The Current Situations & Future Perspective of The Acetone-Butanol Fermentation Industries in ChinaSYTU 3The History of Acetone-ButanolFermentative  Industries in ChinaSYTU 4Strains & Fermentation MaterialsIndustrial Fermentation StrainsFermentation Materials?Corns;?Cassava, Sweet Potato, PotatoStrain Company/Institution (isolated by) Major Characteristics C.acetobutylicumAS 1.70Microbiology Institute of Chinese Science Academy (CSA)C.acetobutylicumNA-2Shanghai Solvent Plant Various excellent phage-resistant propertiesC.acetobutylicumEA 2018Shanghai Institute of Plant Physiology, CSAHigh ratio of butanolcontentSYTU 5Foundation of The Chinese ABE Fermentation IndustriesLocations & Historic Changes of The Chinese Fermentative Solvent Plants1st generation’s plant, 1960-1965 2nd generation’s plant, 1965-1980 3rd generation’s plant, 1980-2000 The Corn Beltin ChinaThe Capacity of The Chinese ABE Fermentation IndustriesTime & PeriodsTotal Capacity(Ton) in The NationCapacity in Single Ordinary Solvent Plants (Ton)Capacity in Solvent Plant with Highest ProductionNumbers of Total Solvent Plants 1960’s N/A 1,000 N/A 4-51965-1970 40,000-50,0003,000 10,000 (Shanghai Solvent Plant)152001-present N/A N/A N/A N/A1980 170,000 5,000-10,000 N/A 281990-2000 0-60,000 N/A N/A 4-5SYTU 7One of The Major ABE Solvent Companies in ChinaHuabei Pharmaceutical Co. Ltd., Shijiazhuang, Hebei ProvinceSYTU 8One of The Major ABE Solvent Companies in ChinaMajor ABE fermentation facilities & Equipment – Fermentation PlantSYTU 9One of The Major ABE Solvent Companies in ChinaMajor ABE fermentation facilities & Equipment – FermentorsSeed TankMain FermentorsSYTU 10One of The Major ABE Solvent Companies in ChinaMajor ABE fermentation facilities & EquipmentCorn Deposit Tower Distillation TowerSYTU 11The Acetone-Butanol Industrial Fermentation Techniques in ChinaSYTU 12The High Butanol Ratio’s Production Strain EA 2018A:B:E=18.2~26.3: 67.6~75.0: 6.4~11.7versus  traditional A:B:E ratio = 3:6:1ABE Fermentation with strain EA2018(Y.Zhang et al., 1996)Solvent concentrations and butanol ratio in batch fermentation with EA2018 series strains (Y.Zhang et al., 1996)The High Butanol Ratio’s Production Strain EA 2018SYTU 14The High Butanol Ratio’s Production Strain EA 2018尚无数据 71.45% vs 58%20.95% vs 30%7.6% vs 12%Batch data of the industrial fermentation using EA 2018 strain, reported by Jizhong Solvent Plant, Hebei Province (Y.Zhang et al., 1996)The High Butanol Ratio’s Production Strain EA 2018The schematic flow chart of continuous ABE fermentation (Z. Sun, 1981) Continuous ABE Fermentation TechniquesThe experimental results of the continuous ABE fermentation (Z. Sun, 1981) Continuous ABE Fermentation TechniquesSYTU 18Multiple stages (6-11 tanks in-series) were set for the optimal cells growth & solvent production, maintaining the cell metabolic activities at highest level. The fresh substrate solution was continuously fed into the first tank together with periodic addition of seed culture broth, assuring strains always at their activated phase.  Productivity increased about 20%, compared with the traditional batch fermentation.  Less strain contamination & mutation risks. Continuous ABE Fermentation TechniquesAdvantages & Unique Behaviors:SYTU 19Products Recovery & Wastes Utilization Techniques? Recovery of solvent contained in exhaust gas (a mixture of A:B:E=55:22:23, about 1-2% of the total solvent amount. ?Recovery of the evolved CO2 and H2 as the by-products into compressed tanks. 36 m3 CO2 & 24 m3 H2 harvested  per 100kg starch.  ?Average solvent production yield per 100kg starch: Acetone 11kg; Butanol22.5kg; Ethanol 2.7kg.?Wastes treatment: to use the waste mash for anaerobic fermentation to get the dried slurry as fertilizer, or for getting DDGS & DDG feedstock. The overall flow chart of the acetone-butanolfermentation from corns (Sun, Z. & Jiao, R., 2003) SYTU 20Repeated Batch ABE Fermentation with Immobilized CellsRepeated batch acetone-butanol fermentation with immobilized cells (Z. Sun et al., 1987) A total of 10 batches, 32 daysSYTU 21Continuous ABE Fermentation with Immobilized CellsCascaded packed column bioreactor (3 in-series) for ABE fermentation (Z. Sun et al., 1988) Conditions:?Immobilization carrier: ceramic rings, ρ: 700g/L, Size: ϕ12x12 mm;?Packed column reactor, 3 in-series: VT=5.18L, Vef=4.66L, carrier packing amount=3.23kg, total broth volume=2.89L; ?Feeding substrate: 5%-8% fresh corn mash; ?Strain: C.acetobutylicum AS 1.70SYTU 22Continuous ABE Fermentation with Immobilized CellsExperimental result of the extremely long-term continuous ABE fermentation with immobilized cells (Z. Sun et al., 1988) SYTU 23Typical Solvents Purification Process, 4 towers fractionation SystemSYTU 24The Current Situation & Future Perspective of The Acetone-ButanolFermentation Industries in ChinaSYTU 25The Problems & Challenges of ABE Fermentation in China?The strong competition from the petrochemical industry, economically;?The rising grain price (RMB1,200[$150]/Ton-corn, much higher than US domestic corn prices;?Low solvent concentrations, yield and productivity; unfavorable solvents contents or ratio; high cost for product purification and wastes treatmentProblems?Severe shortage in oil resources, large amount of crude oil relying on import ⇒seeking substitutable resources;?Energy & material strategies as a country lack of oil, but enriched with biomass, including starches, cellulose, and agricultural/industrial/daily-life wastes;  ?Air & environmental pollution controlChallengesSYTU 26The Future Perspective of ABE Fermentation Industries in China?The severe shortage and price rise-up of oil price forced the government officers, scholars, and enterpriser to face and pay more attention on the development of the fermentative acetone-butanol techniques; ?AB are important products closely related with the national economy and people’s daily life.  The raw materials for fermentative acetone-butanol production are the renewable biomass resources.  The products obtained by green biotechnology and fermentation methods are not only more applicable for the food and pharmaceutical industries, but also playing a key contribution role in relieving the problems of energy shortage & environmental pollution; ?With the development in microbial breeding and fermentation technologies, the traditional ABE fermentation industry still has a strong vitality, and development of ABE fermentative industries in China would have bright future. SYTU 27The Solutions for Modernization of ABE Fermentation?Efficient substrates utilization, including using the agricultural products residuals and waste cellulose as the raw fermentation materials;?Improving strains resistant abilities to metabolites, increasing solvents conc.; ?Modification of fermentation technologies, such as improvement of the performance of the continuous or immobilized cell production processes; ?Improvement of the entire technologies, including comprehensive utilization of by-products, and upgrading waste-water treatment techniques?Fermentation techniques without downstream distillationSYTU 28Southern Yangtze University (SYTU), School of BiotechnologyTop-ranked institution in Fermentation Eng. area of  the nation, specialized inBio-catalytic conversionEnzymatic engineering Industrial microbiologyBio-energy & Bio-resourcesBioreactor engineeringSYTU 29Location of Southern Yangtze UniversityShanghaiNanjingHangzhouWuxiSuzhouTaihu LakeYangtze River DeltaYellow SeaEastern China SeaThe Yangtze RiverSchool of BiotechnologySouthern Yangtze UniversitySYTU 31http:www.sytu.edu.cnThank you, and welcome to visit SYTUProf. Zhihao SunPh.D Advisor,Head of Biocatalysis Laboratory, Southern Yangtze University? Tel / Fax: 86-510-5808498:   ? Email: sunw@public1.wx.js.cn ;sunzhihao@sytu.edu.cn

The Acetone-Butanol (ABE) Fermentation Industries in China

https://dc.engconfintl.org/cgi/viewcontent.cgi?article=1006&amp;context=bioenergy_i

The Acetone-Butanol (ABE) Fermentation Industries in China

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