Optimization of a DNA extraction method from complex samples (pig ́s kidneys) to detect Leptospiraspp

Abstract

Leptospirosis is a zoonotic disease caused by Leptospira spp. In Ecuador this disease is a latent problem that can affect both humans and animals. The relevance of monitoring pathogenic Leptospira spp., specifically in backyard animals, lies in the possible exposure of humans and other animals to this pathogen and contributes to the epidemiological understanding of this disease. Molecular detection, although expensive, is a much more reliable tool for the detection of Leptospira spp.than other types of techniques...La leptospirosis es una enfermedad zoonótica causada por Leptospira spp. En Ecuador esta enfermedad es un problema latente que puede afectar tanto a humanos como a animales. La relevancia del monitoreo de Leptospira spp. patógena, específicamente en animales de traspatio, radica en la posible exposición del ser humano y otros animales a este patógeno y contribuye a la comprensión epidemiológica de esta enfermedad..

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