Inhibition of toxic actions of phospholipase A2 isolated & characterized from the Indian Banded Krait (Bungarus fasciatus) venom by synthetic herbal compounds
Phospholipase A2 (PLA2) is one of the major constituents of krait venom
associated with several pathophysiological actions like myotoxicity, cardiotoxicity, neurotoxicity, 12&6
As there was no specific antiserum available against +,(withsyntheticherbalcompounds,antiPLA2rabbitantiserumandcommercialpolyvalentsnakevenomantiserumtoneutralizethePLA2inducedtoxicitiesinexperimentalmodels.venomphospholipaseA2fraction38(BFβ38)wasisolatedbyionexchangechromatography,molecularweightwasdeterminedbymassspectrometryanditsNterminalaminoacidsequencewasidentified.MonospecificrabbitantiserumwasraisedagainstthePLA2inpresenceofFreundcompleteadjuvant.TheneutralizationofPLA2inducedtoxicitieswasdonein3,432)βandin434β3,modelsusingsyntheticherbalcompounds,antiPLA2rabbitantiserumandcommercialpolyvalentsnakevenomantiserum.AtoxicPLA2(BFβ38)waspurifiedfromthe:7%5&3%2+5 venom by CM-cellulose and HPLC, of 13.17
kDa and a minor band of 7.3 kDa using ESI-MS. The 13.17 kDa PLA2 sequence was NLYQFKNMIQC.
The 7.3 kDa toxin sequence was RKCLTKYSQDNES and was found to be <10 per cent w/w. Anti PLA2
rabbit antiserum produced faint precipitant band in immunogel diffusion and showed low titre value.
The commercial polyvalent snake venom antiserum, anti PLA2 rabbit antiserum and the synthetic herbal
compounds neutralized the PLA2 induced toxicities at different intensities.
Our results suggested that synthetic herbal compound (BA) along with
antiserum might provide effective protection against PLA2 induced toxicities of $:7%5&3%2+5 venom