Bull, L

Cree, S

Dudhia, J

Garvican, E R

Smith, R K W

English

PubMed

Elaine R. Garvican

Sandra Cree

Lydia Bull

Roger K. W. Smith

Jayesh Dudhia

Crossref

ERRATUM Open AccessErratum to: Viability of equinemesenchymal stem cells during transportand implantationElaine R. Garvican, Sandra Cree†, Lydia Bull†, Roger K. W. Smith and Jayesh Dudhia*ErratumFollowing the publication of our article [1] the followingminor errors and omissions were brought to ourattention.IntroductionThroughout the manuscript, reference should have beenmade to Bone Marrow Supernatant (BMS) and not BoneMarrow Aspirate (BMA).Materials and MethodsApoptosis assayAssays using Annexin V and propidium iodide were per-formed on separate samples (replicates) and not as repeatedmeasures, a point that was not made clear in the originalarticle. Separate aliquots of cells for each time point werekept in culture, without media changes, until assayed.Cell proliferation assay and Metabolic activityIn alamarBlue® experiments relating to transport media,we used the interpretation of proliferation because cellswere cultured over several days, allowing time for cellproliferation to take place. In contrast, when interpretingviability assays following needle extrusion, we employeda metabolic activity interpretation because the cells werecultured for a short period (24 h, Fig. 5) when cell prolif-eration would not be a prominent feature. Measure-ments in both instances were of fluorescence.ResultsFigure 3: The Key was accidently omitted. The correctfigure is provided here (Fig. 3).Figure 5: The y-axis is incorrectly labelled as “Absorb-ance”. The correct axis label should read “% non-injectedcontrol” and is based on fluorescence readings. The cor-rect figure is provided here (Fig. 5).Where appropriate, all figure legends in our articleshould state that error bars represent the standard errorof the mean (± SEM).These corrections do not alter the results or conclusionof the study that (i) the medium used for transportation ofbone marrow mesenchymal stem cells had a significantimpact on cell viability with increasing transport time and(ii) the use of a larger needle size (19G) for cell implant-ation reduced stress-induced loss of viability compared tosmaller needle gauges (21G and 23G).* Correspondence: jdudhia@rvc.ac.uk†Equal contributorsThe Royal Veterinary College, Clinical Sciences and Services, Hawkshead Lane,North Mymms, Hatfield AL9 7TA, UK© The Author(s). 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, andreproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link tothe Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Garvican et al. Stem Cell Research & Therapy  (2016) 7:161 DOI 10.1186/s13287-016-0423-zFig. 3 Cell proliferation measured by alamarBlue™ assay for mesenchymal stem cells (MSCs) (n = 3) following storage for 24 or 72 h in suspensionmedia. a Initial proliferation rates; b Change in the proliferation rate with time following storage for 24 h; c Change in the proliferation rate withtime following storage for 72 h. BMA, bone marrow aspirate; D10, Dulbecco’s Modified Eagle Medium supplemented with 10 % fetal bovineserum; PRP, platelet rich plasma; HA, hyaluronic acid. Error bars represent the standard error of the mean (± SEM)Garvican et al. Stem Cell Research & Therapy  (2016) 7:161 Page 2 of 3Received: 12 October 2016 Accepted: 14 October 2016References1. Garvican ER, Cree S, Bull L, Smith RKW, Dudhia J. Viability of equinemesenchymal stem cells during transport and implantation. Stem Cell ResTherapy. 2014;5:94.Fig. 5 Analysis of early and late apoptosis and cell metabolic activity after injection through different gauge needles. Data is shown for viabilitymeasured immediately after injection of mesenchymal stem cells (n =3) in D10 through 19G, 20G or 21G needles and in non-injected controls(NI). a) Annexin V positive cells expressed as a percentage of the total cell population; b propidium iodide-positive cells expressed a percentageof the total cell population; c metabolic activity (fluorescence) of mesenchymal stem cells measured by alamarBlue™ assay. Error bars representthe standard error of the mean (± SEM)Garvican et al. Stem Cell Research & Therapy  (2016) 7:161 Page 3 of 3

Stem Cell Research & Therapy

Erratum to: Viability of equine mesenchymal stem cells during transport and implantation

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ERRATUM Open Access
Erratum to: Viability of equine
mesenchymal stem cells during transport
and implantation
Elaine R. Garvican, Sandra Cree†, Lydia Bull†, Roger K. W. Smith and Jayesh Dudhia*
Erratum
Following the publication of our article [1] the following
minor errors and omissions were brought to our
attention.
Introduction
Throughout the manuscript, reference should have been
made to Bone Marrow Supernatant (BMS) and not Bone
Marrow Aspirate (BMA).
Materials and Methods
Apoptosis assay
Assays using Annexin V and propidium iodide were per-
formed on separate samples (replicates) and not as repeated
measures, a point that was not made clear in the original
article. Separate aliquots of cells for each time point were
kept in culture, without media changes, until assayed.
Cell proliferation assay and Metabolic activity
In alamarBlue® experiments relating to transport media,
we used the interpretation of proliferation because cells
were cultured over several days, allowing time for cell
proliferation to take place. In contrast, when interpreting
viability assays following needle extrusion, we employed
a metabolic activity interpretation because the cells were
cultured for a short period (24 h, Fig. 5) when cell prolif-
eration would not be a prominent feature. Measure-
ments in both instances were of fluorescence.
Results
Figure 3: The Key was accidently omitted. The correct
figure is provided here (Fig. 3).
Figure 5: The y-axis is incorrectly labelled as “Absorb-
ance”. The correct axis label should read “% non-injected
control” and is based on fluorescence readings. The cor-
rect figure is provided here (Fig. 5).
Where appropriate, all figure legends in our article
should state that error bars represent the standard error
of the mean (± SEM).
These corrections do not alter the results or conclusion
of the study that (i) the medium used for transportation of
bone marrow mesenchymal stem cells had a significant
impact on cell viability with increasing transport time and
(ii) the use of a larger needle size (19G) for cell implant-
ation reduced stress-induced loss of viability compared to
smaller needle gauges (21G and 23G).
* Correspondence: jdudhia@rvc.ac.uk
†Equal contributors
The Royal Veterinary College, Clinical Sciences and Services, Hawkshead Lane,
North Mymms, Hatfield AL9 7TA, UK
© The Author(s). 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Garvican et al. Stem Cell Research & Therapy  (2016) 7:161 
DOI 10.1186/s13287-016-0423-z
Fig. 3 Cell proliferation measured by alamarBlue™ assay for mesenchymal stem cells (MSCs) (n = 3) following storage for 24 or 72 h in suspension
media. a Initial proliferation rates; b Change in the proliferation rate with time following storage for 24 h; c Change in the proliferation rate with
time following storage for 72 h. BMA, bone marrow aspirate; D10, Dulbecco’s Modified Eagle Medium supplemented with 10 % fetal bovine
serum; PRP, platelet rich plasma; HA, hyaluronic acid. Error bars represent the standard error of the mean (± SEM)
Garvican et al. Stem Cell Research & Therapy  (2016) 7:161 Page 2 of 3
Received: 12 October 2016 Accepted: 14 October 2016
References
1. Garvican ER, Cree S, Bull L, Smith RKW, Dudhia J. Viability of equine
mesenchymal stem cells during transport and implantation. Stem Cell Res
Therapy. 2014;5:94.
Fig. 5 Analysis of early and late apoptosis and cell metabolic activity after injection through different gauge needles. Data is shown for viability
measured immediately after injection of mesenchymal stem cells (n =3) in D10 through 19G, 20G or 21G needles and in non-injected controls
(NI). a) Annexin V positive cells expressed as a percentage of the total cell population; b propidium iodide-positive cells expressed a percentage
of the total cell population; c metabolic activity (fluorescence) of mesenchymal stem cells measured by alamarBlue™ assay. Error bars represent
the standard error of the mean (± SEM)
Garvican et al. Stem Cell Research & Therapy  (2016) 7:161 Page 3 of 3


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ERRATUM TO: VIABILITY OF EQUINE MESENCHYMAL STEM CELLS DURING TRANSPORT AND IMPLANTATION

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ERRATUM TO: VIABILITY OF EQUINE MESENCHYMAL STEM CELLS DURING TRANSPORT AND IMPLANTATION

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