Bond, R

Chang, Y M

Clark, S M

Loeffler, A

Schmidt, V M

Timofte, D

Wilson, A

English

Siân‐Marie Clark

Anette Loeffler

Vanessa M Schmidt

Yu‐Mei Chang

Alison Wilson

Dorina Timofte

Ross Bond

Crossref

Veterinary Dermatology

Interaction of chlorhexidine with tris<scp>EDTA</scp>or miconazolein vitroagainst canine meticillin‐resistant and ‐susceptibleStaphylococcus pseudintermediusisolates from two<scp>UK</scp>regions

BackgroundTopical therapy is an important alternative to systemic antibacterial therapy for treatment of canine superficial pyoderma in light of the emergence of multidrug-resistant staphylococci. Chlorhexidine is widely used in shampoo products alone or in combination with miconazole or tromethamine-ethylenediaminetetraacetic acid (trisEDTA). Comparisons of these combinations have not been made.Hypothesis/objectivesTo determine minimum inhibitory concentrations (MICs) of combinations of chlorhexidine/miconazole and chlorhexidine/trisEDTA in vitro in a collection of Staphylococcus pseudintermedius (SP) from northern (NUK) and southeastern (SEUK) United Kingdom (UK) sources.MethodsMICs of chlorhexidine, miconazole, trisEDTA and combinations of chlorhexidine/miconazole (1:1) or chlorhexidine/trisEDTA (80:16:1 and 80:5:1) were determined for 196 canine SP isolates from NUK [49 meticillin-resistant (MRSP), 50 meticillin-susceptible (MSSP)] and fom SEUK (48 MRSP, 49 MSSP) using agar dilution.ResultsTrisEDTA alone did not inhibit growth. Chlorhexidine/miconazole MICs (median = 0.5 mg/L) were lower than those of either drug alone (P Conclusions and clinical importanceThese low MICs are likely to be exceeded by topical therapy. Evaluation of the mechanisms by which chlorhexidine combinations interact to reduce MICs is warranted, in view of increasing concerns of biocide tolerance in staphylococci

Clark, Sian-Marie

Loeffler, Anette

Schmidt, Vanessa M

Chang, Yu-Mei

Wilson, Alison

Timofte, Dorina

Bond, Ross

University of Liverpool Repository

Interaction of chlorhexidine with trisEDTA or miconazole in vitro against canine meticillin-resistant and -susceptible Staphylococcus pseudintermedius isolates from two UK regions

RVC Research Online

Interaction of chlorhexidine with trisEDTA or miconazole in vitro against canine meticillin-resistant and susceptible Staphylococcus pseudintermedius isolates from two UK regions

https://researchonline.rvc.ac.uk/id/eprint/10181/1/10181.pdf

Interaction of chlorhexidine with trisEDTA or miconazole in vitro against canine meticillin-resistant and susceptible Staphylococcus pseudintermedius isolates from two UK regions

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University of Liverpool Repository

RVC Research Online