Azinheira, H.

Batista, D.

Santos, D.

Silva, M.C.

Talhinhas, P.

Tavares, S.

Várzea, V.

English

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www.alphavisa.com/asic/2021Posters  - Session 2 - Posters Session 2: Plant pathology & protection25328th Conference of Association for the Science and Information on Coffee28 June - 1 July 2021 Montpellier, FranceBook of abstractsS2-P-27Identification of NLR proteins in the coffee genotype HDT 8232/2 challenged with Hemileia vastatrix (host resistance) and Uromyces vignae (nonhost resistance)Tavares Silvia1 (sagtavares@gmail.com), Azinheira Helena1, Santos Diogo2, Batista Dora1, Varzea Vitor1, Talhinhas Pedro3, Silva Maria do Ceu11 CIFC, LEAF, Instituto Superior de Agronomia, Oeiras, Portugal ; 2 IGC, Oeiras, Portugal ; 3 CIFC, LEAF, Instituto Superior de Agronomia, Lisboa, PortugalRATIONALEThe natural hybrids between Coffea arabica and Coffea canephora, known as “Timor Hybrids - HDTs”, have been used as the main source of resistance to leaf rust (Hemileia vastatrix) in coffee breeding programs. However, the molecular nature of the resistance depicted by the HDTs remains elusive. The identification of resistance proteins, specifically NRL (Nucleotide-binding and Leucine-Rich proteins) that are responsible for the immunity triggered by effectors is of major importance and NLR-coding resistance genes are widely used to protect crops against many diseases.METHODSWe used three cDNA libraries generated by highthroughput sequencing of RNA from leaves of the Timor Hybrid HDT 832/2; non-inoculated (control), inoculated with H. vastatrix (incompatible interaction) and inoculated with Uromyces vignae (nonhost interaction) (Diniz et al. 2012). The sequences obtained were analyzed by a bioinformatic pipeline aiming to identifying NLR proteins. In recent years, the genomes of C. canephora, C. arabica (Denoeud et al. 2014) and C. eugenioides were sequenced, which allowed us to compare the NLR from the three different species with the sequences obtained from the HDT transcriptome.RESULTSWe could identify almost one hundred putative NLRs proteins in the transcriptome libraries that showed the conserved domains of NLRs protein. The majority was present in all cDNAs libraries, a small group was only detected in inoculated leaves, and an equally restricted group was present in the control and in the nonhost interaction. Interestingly, the upregulated sequences in leaves inoculated with H. vastatrix (incompatible interaction) were equally upregulated in leaves inoculated with U. vignae (nonhost interaction).CONCLUSIONS & PERSPECTIVESNLRs are extremely abundant in plant genomes, for instance in C. canephora genome more than seven hundred different proteins sequences were predicted. This high number of NLRs is reflected in the transcriptome of the HDT 832/2, however most of the sequences seem to have an unaltered transcription level when compared to the control. These findings reveal the diversity of NLRs and the existence of specific groups for the different interactions. We intend to identify NLR in other coffee genotypes with high spectrum of resistance to H. vastatrix, such as HDT derivatives and the Kawisari hybrid (C. arabica x C. liberica), under our new project CoffeeRES, to unveil the role of these proteins in the coffee immune system.Acknowledments: This work was funded with national funds through Foundation for Science and Technology (FCT) and FEDER funds through PORNorte under the project Project CoffeeRES ref. PTDC/ASP-PLA/29779/2017, and FCT UNIT LEAF (UID/AGR/04129/2020).References:• Denoeud et al. 2014 Science DOI: 10.1126/science.1255274• Diniz et al. 2012 Eur J Plant Pathol DOI: 10.1007/s10658-011-9925-9

Identification of NLR proteins in the coffee genotype HDT 8232/2 challenged with Hemileia vastatrix (host resistance) and Uromyces vignae (nonhost resistance)

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Identification of NLR proteins in the coffee genotype HDT 8232/2 challenged with Hemileia vastatrix (host resistance) and Uromyces vignae (nonhost resistance)

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