ABSTRACT: Chlorinated olefins were formed In vitro from hexachloroethane, pentachloroethane, and 1,1,1,2-tetrachloroethane by phenobarbitalinduced rat liver microsomes. The production of tetrachloroethylene, trichloroethylene, and 1,1-dichloroethylene, respectively, was quantified by gas chromatographic analysis of headspace samples from the reaction vessels. The reaction showed a pH optimum of 7.0-7.5 under a nitrogen atmosphere; oxygen inhibited the reaction