18 O SO4 isotopic signatures of the mine drainage and the presence of presumptive SO 4 -reducing bacteria suggest that SO 4 reduction activity also contributes C depleted in 13 C isotope to the total DIC pool. With distance downstream from the mine portal, C isotope signatures in the drainage increased , accompanied by decreased total DIC concentrations and increased pH. These data are consistent with H 2 SO 4 dissolution of carbonate rocks, enhanced by cation exchange, and C release to the atmosphere via CO 2 outgassing