ABSTRACT The Solanum trilobatum Linn. (Family: Solanacea) root powder was extracted with chloroform, methanol and screened for its anti-microbial activity against various gram (+) ve, gram (-) ve bacteria and fungal organisms using cup plate agar diffusion method. The results revealed that the chloroform and methanolic extracts shown significant anti-microbial activity at concentrations of 100 mg/ml and 200 mg/ml against tested organisms, particularly these were effective against gram (+)ve bacteria Bacillus subtilis, Staphylococcus aureus, gram (-)ve bacteria Escherichia coli and fungi Candida albicans, Saccharomycis cerevisicea