Abstract The presence of mycoplasma in ovine semen was associated in 9.09% (3/ 33) to the microscopic and macroscopic alterations of this fluid. Mycoplasma spp. was isolated in 36.36% (12/33) fro m semen samples while Ureaplasma spp. was isolated in 12.12% (4/ 33). The mo llicute infection rates in studied semen samples indicated that the diagnosis of these bacteria in ovine must be a routine procedure for the quality of this biological product
