Abstract Aims: The focus of this study was to assess the production of secondary metabolites of callus derived from Jatropha curcas L. petiole explants. Results: Excellent growth of callus was obtained. Callus was soft, friable, lush green in color and grew fast from 8 to 30 days of culture then stabilized at low growth rate. The results obtained by phyto-chemical tests revealed the presence of saponins, tannins, alkaloids, steroids and flavonoids. The tannin contents in vitro proliferated callus, leaves, stem barks, root barks, roots and latex of J. curcas were found to be 161, 173, 220, 214, 43 and 245 μg tannic acid equivalent/g of dry weight respectively. Conclusion: The results of this study have revealed that in vitro induced callus from J. curcas petiole explants was able to produce secondary metabolites. The pharmacological activity of J.curcas reported by various researchers can be attributed to the presence of these phytochemicals
