Purpose: To compare the central corneal thickness (CCT) measurements of patients with neovascular age-related macular degeneration (AMD) and control subjects. Methods: The CCT value (measured with ultrasound corneal pachymetry) of 130 eyes (130 patients, 1 eye from each patient) with neovascular AMD (AMD group) and 98 eyes (98 patients, 1 eye from each patient) of similar age, sex, and eye&apos;s axial length healthy control subjects (normal group) was compared. Results: The mean age (AMD group: 69.1 years vs. control group: 69.5 years, P = 0.81), sex (AMD group: 77 women, 59% vs. control group: 59 women, 60%, P = 0.77), and eye&apos;s axial length (AMD group: 25.05-mm vs. control group: 24.61-mm, P = 0.38) of patients with neovascular AMD and healthy control subjects were comparable. There were no statistically significant differences in the mean CCT measurements in the neovascular AMD group in comparison with the control group (549.44 vs. 544.35 mm, P = 0.11). Conclusions: CCT measurements do not differ in patients with neovascular AMD compared with healthy control subjects

MD Emmanouel Christodoulakis

MD George C Hajithanasis

MD George D Kymionis

MD Ioannis G Pallikaris

MD Miltiadis K Tsilimbaris

MD Sonia H Yoo

MD Theoni D Panagiotoglou

MD, MSc Nikolaos S Tsiklis

CiteSeerX

CLINICAL SCIENCE
Central Corneal Thickness in Patients With Neovascular
Age-Related Macular Degeneration
George D. Kymionis, MD, PhD,*† Theoni D. Panagiotoglou, MD,* Sonia H. Yoo, MD,†
Nikolaos S. Tsiklis, MD, MSc,* Emmanouel Christodoulakis, MD,* George C. Hajithanasis, MD,*
Miltiadis K. Tsilimbaris, MD, PhD,* and Ioannis G. Pallikaris, MD, PhD*
Purpose: To compare the central corneal thickness (CCT)
measurements of patients with neovascular age-related macular
degeneration (AMD) and control subjects.
Methods: The CCT value (measured with ultrasound corneal
pachymetry) of 130 eyes (130 patients, 1 eye from each patient) with
neovascular AMD (AMD group) and 98 eyes (98 patients, 1 eye from
each patient) of similar age, sex, and eye’s axial length healthy control
subjects (normal group) was compared.
Results: The mean age (AMD group: 69.1 years vs. control group:
69.5 years, P = 0.81), sex (AMD group: 77 women, 59% vs. control
group: 59 women, 60%, P = 0.77), and eye’s axial length (AMD
group: 25.05-mm vs. control group: 24.61-mm, P = 0.38) of patients
with neovascular AMD and healthy control subjects were compa-
rable. There were no statistically significant differences in the mean
CCT measurements in the neovascular AMD group in comparison
with the control group (549.44 vs. 544.35 mm, P = 0.11).
Conclusions: CCT measurements do not differ in patients with
neovascular AMD compared with healthy control subjects.
Key Words: corneal thickness, neovascular, age-related macular
degeneration
(Cornea 2007;26:182–184)
Age-related macular degeneration (AMD) is the leadingcause of blindness in the developed world.1,2 The etiology
of AMD is poorly understood, but it is most likely a complex
disease in which several risk factors seem to have a potential
role.3 Smoking, hyperlipidemia, elevated blood pressure, and
atherosclerosis and history of cardiovascular diseases are a
few of the described risk factors that may contribute in
the development of this disease.4,5
Central corneal thickness (CCT) is of great significance
clinically. It has been associated with several systemic ocular
conditions such as active Behcxet disease, Down syndrome,
diabetes, osteogenesis imperfecta, keratoconus, dry eye,
glaucoma, and retinal detachment.6–13 It has been hypothe-
sized that ocular factors, such as cataract extraction, iris color,
refractive errors, and ocular rigidity, may also be involved in
the development of AMD,14,15 whereas there is a possible
correlation between these factors and CCT (CCT contributes
to the corneal rigidity that is 1 of the major components of
ocular rigidity besides scleral rigidity). On the basis of these
observations, we compared the CCT of patients with
neovascular AMD and control subjects to study the possible
association of CCT with AMD.
MATERIALS AND METHODS
Ninety-eight eyes of 98 healthy subjects (control group,
59 women, 60%) and 130 eyes of 130 patients previously
diagnosed with AMD with choroidal neovascularization
(AMD group: 77 women, 59%) were included in this
prospective study. The institutional review board at the
University of Crete approved the study protocol. All study
procedures adhered to the Declaration of Helsinki for
research involving human subjects. After informed consent
was obtained, a complete ocular examination was performed.
The ophthalmic examination included a measurement of
visual acuity, slit-lamp examination, measurement of CCT,
indirect and direct ophthalmoscopy, and fundus photography.
Exclusion criteria were the use of contact lens, any corneal
pathology, eye drop users, dry eye symptoms, previous
ophthalmic surgery (except photodynamic therapy for corneal
neovascularization (CNV) patients), history of corneal disease,
and ocular trauma. Patients were classified as having
neovascular AMD on the basis of standard findings by clinical
examination and fluorescein angiography. Neovascular AMD
was defined as the presence of a serous or hemorrhagic
neuroretinal or retinal pigment epithelium (RPE) detachment
and/or a subretinal neovascular membrane and/or a subretinal
hemorrhage and/or a periretinal fibrous scar. No distinction
was made about the type or stage of CNV (ie, classic, occult,
retinal angiomatous proliferation, or disciform scar forma-
tion). Patients may have undergone treatment of their CNV.
Lesions that were considered to be the result of generalized
disease, such as diabetic retinopathy, chorioretinitis, high
myopia, trauma, congenital diseases, or photocoagulation for
Received for publication April 11, 2006; revision received October 15, 2006;
accepted October 17, 2006.
From the *Department of Ophthalmology, Institute of Vision and Optics,
University of Crete, Crete, Greece; and the †Bascom Palmer Eye Institute,
University of Miami, Miami, FL.
The authors state that they have no proprietary interest in the products named
in this article.
Reprints: George D. Kymionis, University of Crete Medical School,
Department of Ophthalmology, 71110 Heraklion, Crete, Greece (e-mail:
kymionis@med.uoc.gr).
Copyright  2007 by Lippincott Williams & Wilkins
182 Cornea  Volume 26, Number 2, February 2007
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reasons other than neovascular AMD, were excluded. The
control group included patients who were examined for routine
cataract extraction without evidence of drusen, pigmentary
changes, CNV, or any other pathologic ocular condition
(except cataract), whereas the AMD group included patients
with neovascular AMD who were treated with photodynamic
therapy.
CCT, in both healthy volunteers and AMD, was
evaluated by the same experienced ophthalmologist (N.T.)
with ultrasound pachymetry (DGH 5100 Technology, Exton,
PA). Corneal thickness measurements were carried out from
10:00 AM to 12:00 AM to avoid the diurnal variation in corneal
thickness values and before any invasive examination pro-
cedure (such as Goldmann tonometry). Corneal anesthesia was
achieved using benoxinate 0.4% applied immediately before
measurement. The probe tip of the pachymeter was held
perpendicular to the cornea and centered over the pupil. Each
patient was asked to blink before CCT measurements to avoid
any bias because of corneal drying. Five consecutive
measurements were made at the center of the cornea of each
eye. The lowest CCT measurement was used in the statistical
analysis because it was thought to most likely reflect a
perpendicular placement of the pachymeter probe and there-
fore to be the most accurate measurement.
Unpaired Student t test was applied. P values less than
0.05 were considered to be statistically significant.
RESULTS
Table 1 presents the demographic, clinical, and ocular
characteristics of the study cohort of study participants. The
mean age in healthy subjects was 69.5 6 9.7 (SD) years
(range, 51–90 years) and was 69.1 6 11.5 years (range, 44–
83 years) in patients with AMD. There were no statistically
significant differences in age between study groups (P = 0.81).
Furthermore, the 2 study groups were comparable for sex
(control group: 59 women, 60% vs. AMD group: 77 women,
59%, P = 0.77) and ocular axial length [control group:
24.61 6 2.75 mm (range, 21.61–31.00 mm) vs. AMD group:
25.05 6 2.78 mm (range, 22.06–31.02 mm); P = 0.38; Table 1].
The mean CCT measurements were not significantly dif-
ferent in the neovascular AMD group compared with the
control group [549.44 6 26.41 mm (range, 491–601 mm) vs.
544.35 6 18.70 mm (range, 482–586 mm), respectively;
P = 0.11; Table 1]. The mean difference [95% confidence
interval (CI) of the difference] of CCT measurements between
patients with AMD and the control group was 5.09 mm
(95% CI, –1.23 to 11.41).
DISCUSSION
Corneal thickness could be representing a biomechanical
parameter of the eye with several implications. An association
between CCT and several clinical conditions has been
documented (such as optic nerve drusen, pituitary adenoma,
pregnancy, osteogenesis imperfecta, glaucoma, diabetes, and
retinal detachment).6–13,16
Several ocular factors, such as cataract (extraction), iris
color, refractive errors, and ocular rigidity, in addition to
smoking, atherosclerosis, and genetic factors, may also be
involved in the development of AMD.1–5,14,15 The possible
pathophysiologic mechanism by which corneal thickness may
be implicated in the pathogenesis of choroidal neovasculari-
zation in AMD could be the correlation between corneal
thickness and corneal elasticity, scleral stiffness, and ocular
rigidity17 (a significant risk factor for the development of
AMD according to the Friedman theory18,19). It was proposed
that the rigid and noncompliant sclera limits the filling of
the vortex veins and thereby increases choroidal vascular
resistance. The final result of this cascade is that there is
a decompensation of the choroidal venous system at the
posterior pole, the Bruch membrane, and the retinal pigment
epithelium of the macular area, leading to the development
of AMD.
In this study, we did not find that patients with
neovascular AMD had differences in CCT measurements
compared with healthy control subjects. Furthermore, in
a recently published article,20 we found no statistically
significant correlation between ocular rigidity coefficient
and CCT. However, as the authors state, this finding cannot
be considered conclusive because the power to detect such
a correlation was low (type II error = 0.64), and the mea-
surements were made after cataract extraction (increasing the
variability of the measurements caused by induced alterations
in endothelial cells).
A few potential limitations are apparent in this study.
The possible impact of photodynamic therapy that most of the
patients with AMD had undergone and the possible existence
of subclinical dry eye (patients with symptoms of dry eye were
excluded) are the major limitations of this study. Furthermore,
even though we could not rule out a difference as large as
11.41 mm (mean difference, 5.09 mm; 95% CI, –1.23 to
11.41), it seems that there was not any clinically meaningful
difference (as has been described in the literature from several
previous studies21–24) between the 2 studied groups.
In conclusion, despite the described limitations of the
study, patients with neovascular AMD do not seem to have
differences in CCT measurements compared with healthy
control subjects.
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TABLE 1. Baseline Characteristics of the Study Participants
Characteristics
AMD Group Control Subjects
P(130 Patients) (98 Patients)
Age (yr; mean 6 SD) 69.1 6 11.5 69.5 6 9.7 0.81
Female [no. (%)] 77 (59%) 59 (60%) 0.77
Axial length (mm; mean 6 SD) 25.05 6 2.78 24.61 6 2.75 0.38
CCT (mm; mean 6 SD) 549.44 6 26.41 544.35 6 18.70 0.11
q 2007 Lippincott Williams & Wilkins 183
Cornea  Volume 26, Number 2, February 2007 CCT in Patients With Neovascular AMD
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Central Corneal Thickness in Patients With Neovascular Age-Related Macular Degeneration

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Central Corneal Thickness in Patients With Neovascular Age-Related Macular Degeneration

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