Streilein, “In vitro generation of regulatory CD8+ T cells similar to those found in mice with anterior chamber-associated immune deviation

Abstract

PURPOSE. When injected intravenously into naïve mice, peritoneal exudate cells (PECs) incubated with ovalbumin (OVA) in the presence of transforming growth factor (TGF)-␤2 induce immune deviation similar to that evoked by injection of OVA into the anterior chamber of the eye. Intraocular antigen injection elicits two distinct populations of regulatory T cells that impair delayed hypersensitivity (DH) by two different mechanisms: a CD4 ϩ T cell that suppresses the induction of DH (afferent) and a CD8 ϩ T cell that inhibits DH expression. In an effort to understand the origin and mechanism of action of these regulatory cells, CD8 ϩ T cells from OVA-specific T cell receptor (Tcr) transgenic mice (OT-1) were used