ABSTRACT Bombyx mori. L. (CSR 2 XCSR 4 ) is a bivolitne crossbreed that produces high quantity of silk. The weights of worms and glands are directly related to the yield of silk, higher larval weights leads to higher silk production. In the present study, quantitative parameters of 5 th instar and pupal stages of silkworm larvae were observed. The analysis of quantitative estimation showed that the maximum weights and lengths of silk gland and silk worm were recorded on 7 th day of 5 th instar larval stage. The day dependent variation in protein concentration was observed in total silk gland 5 th instar larval and pupal stages