Abstract: In this study, the analgesic and anti-inflammatory activities of the methanolic leaf extract of Rothmannialongiflora were investigated. The methanolic leaf extract of the plant at the doses of 250, 500, and 1000mg/kg orally was evaluated for analgesic activity using acetic acid-induced writhing test, hot plate test and formalin test. The anti-inflammatory potential of the extract was evaluated using carrageenan-induced paw oedema test in rats. The methanolic leaf extract exhibited significant dose dependent analgesic and antiinflammatory effects comparable to piroxicam (20mg/kg body weight orally), which is a standard analgesic and anti-inflammatory drug. The preliminary phytochemical screening of the methanolic leaf extract of Rothmannialongiflora revealed the presence of flavonoids, tannins, saponins, glycosides, steroids/terpanoids, and carbohydrates. The oral median lethal dose (LD 50 ) of the extract in mice and rats were found to be greater than 5000mg/kg, suggesting that the methanolic leaf extract of Rothmannialongiflora is non-toxic. The present findings suggest that the methanolic leaf extract of the plant possesses analgesic and anti-inflammatory activities, and supports the ethno medical claims of the use of the plant in the management of pain and inflammatory conditions
