Abstract We introduce BioKIT, a new Hidden Markov Model based toolkit to preprocess, model and interpret biosignals such as speech, motion, muscle and brain activities. The focus of this toolkit is to enable researchers from various communities to pursue their experiments and integrate real-time biosignal interpretation into their applications. BioKIT boosts a flexible two-layer structure with a modular C++ core that interfaces with a Python scripting layer, to facilitate development of new applications. BioKIT employs sequence-level parallelization and memory sharing across threads. Additionally, a fully integrated error blaming component facilitates in-depth analysis. A generic terminology keeps the barrier to entry for researchers from multiple fields to a minimum. We describe our onlinecapable dynamic decoder and report on initial experiments on three different tasks. The presented speech recognition experiments employ Kaldi [1] trained deep neural networks with the results set in relation to the real time factor needed to obtain them
