V.: Regionally-selective adhesion and growth of human osteoblast-like MG 63 cells on micropatterned fullerene C60 layers

Abstract

Fullerenes C60 were deposited on to microscopic glass coverslips using the Leybold Univex-300 vacuum system through metallic masks with rectangle openings. Below the openings, the C60 formed prominences 128 ± 8 nm (A), 238 ± 3 nm (B), 326 ± 5 nm (C) or 1043 ± 57 nm (D) in height. Human osteoblast-like MG 63 cells in the cultures on samples A, B and C were distributed almost homogeneously, while on samples D, they were localized almost exclusively in the grooves among the prominences. Thus, fullerene C60 films can act as substrates for guided cell adhesion and growth