A peptide derived from activity-dependent neuroprotective protein (ADNP) ameliorates injury response in closed head injury in mice

Abstract

ABSTRACT Brain injury induces disruption of the blood-brain barrier, edema, and release of autodestructive factors that produce delayed neuronal damage. NAPSVIPQ (NAP), a femtomolaracting peptide, is shown to be neuroprotective in a mouse model of closed head injury. NAP injection after injury reduced mortality and facilitated neurobehavioral recovery (P Ͻ 0.005). Edema was reduced by 70% in the NAP-treated mice (P Ͻ 0.01). Furthermore, in vivo magnetic resonance imaging demonstrated significant brain-tissue recovery in the NAP-treated animals. NAP treatment decreased tumor necrosis factor-␣ levels in the injured brain and was shown to protect pheochromocytoma (PC12 cells) against tumor necrosis factor-␣-induced toxicity. Thus, NAP provides significant amelioration from the complex array of injuries elicited by head trauma