Molecular and Cellular Pathobiology Altered Dynamics of Intestinal Cell Maturation in Apc

Abstract

Abstract Novel imaging of active transcription sites in interphase nuclei of intestinal epithelial cells in situ showed that key genes associated with Wnt and Notch signaling were dynamically regulated as the cells underwent normal maturation during their migration along the mouse crypt-villus axis (CVA). However, oscillating patterns of activation of these genes were displaced along this axis in the histologically normal intestinal mucosa of Apc 1638N/+ mice before tumor development. Gene expression profiling then showed that the normal repro