ARPN Journal of Agricultural and Biological Science EVALUATION OF THE EFFECT OF MS MEDIUM LEVELS ON ROOTING IN MICRO CUTTINGS OF TEA (Camellia sinensis L.) UNDER IN-VITRO CULTURE CONDITION

Abstract

ABSTRACT Tea [Camellia sinensis (L.)] is one of the most important beverage crops in the Iran. There has been a steady demand for production and supply of cuttings of different varieties for tea gardens in Iran. In order to study the effects of MS medium levels (full MS, half MS and redundancy MS) with 1mg/L indole-3-butyric acid (IBA) hormone on rooting in micro cuttings of tea, an experiment as RCBD with four replications was conducted during 2010 at Research Laboratory of Faculty of Agriculture, Lahijan University in Iran. The culture bed were contained MS levels (full MS, half MS and redundancy MS), 1mg/L IBA hormone, sucrose (3%) and agar (75%). The results show that MS medium levels with IBA hormone on root length and root number in micro cuttings of tea had a significant difference in 1 % probability level. The highest root length (17.22 mm) and root number (4.93) in micro cuttings of tea were obtained with application redundancy MS medium (25% macro element) and 1mg/L IBA. The lowest root length (3.17 mm) and root number (0.68) in micro cuttings of tea were obtained with application full MS medium (25% macro element) and 1mg/L IBA