Abstract. The factors of learning strategies and motivation as components of self-regulation are determined well in MSLQ (Motivated Strategies for Learning Questionnaire) scale. The scale includes 15 factors. But, these 15 factors are not appropriate for practical use. In this study, a survey research was conducted with 112 prospective biology teachers by using MSLQ to analyze the factors. One-way ANOVAs and Pearson Product-Moment Correlation Coefficients were used for analysis. The results of ANOVAs showed that there were no statistically significant differences between mean scores of the participants at different grade levels on scales of MSLQ. In addition, the "task value", the "self-efficacy" and the "help seeking" subfactors were found to be the most dominant sub-factors of the whole scale. In the article, results of the study and limitations will be discussed