Establishment and Characterization of Immortalized Neuronal Cell Lines Derived From the Spinal Cord of Normal and Trisomy 16 Fetal Mice, an Animal Model of Down Syndrome
We report the establishment of continuously growing cell lines from spinal cords of normal and trisomy 16 fetal mice. We show that both cell lines, named M4b (derived from a normal animal) and MTh (trisomic) possess neurological markers by immunohistochemistry (neuron specific enolase, synaptophysin, microtubule associated protein-2 [MAP-2], and choline acetyltransferase) and lack glial traits (glial fibrillary acidic protein and S10
