ANTIOXIDANT, ANTIPLATELET AND CYTOTOXIC ACTIVITY OF EXTRACT OF CYSTOSEIRA AMENTACEA FROM THE COAST OF MONTENEGRO (SOUTH-EAST ADRIATIC SEA)

Abstract

Seaweeds are an excellent source of compounds with biological activity. Since algae have been used in traditional medicine for a long time and also some algal substances have shown cytotoxic, anticoagulant and antiplatelet activity, they have been extensively studied. We examined the cytotoxicity and cell cycle distribution of acetone extract of Brown alga Cystoseira amentacea, on different human malignant cell lines. The antioxidant and antiplatelet activity of the extract has been evaluated. The results indicated that extract of Cystoseira amentacea significantly decreased cell survival in all tested cell lines. The IC50 values in the MTT assay in human cervix carcinoma (HeLa), two human breast cancer cell lines (MDA-MB-361 and MDA-MB-453) and a human colon carcinoma (LS174) cells were ranged from 22.87±1.26 to 64.34±2.78µg/ml. In vitro antitumor activites was accompained by an important sub-G1 accumulation of all cells after treatment of tested cell lines with extract. Antioxidant activity of Cystoseira amentacea extract in vitro was shown using chemically and cell-based assays, in erythrocytes and neutrophils. Finally, extract of Cystoseira amentacea showed significant in vitro inhibitory effects on both platelet activation and aggregation with other blood cells in response to agonist in healthy donors