ABSTRACT A survey study was conducted on some heavy metals (Pb, Cd, Cu, Zn, and Fe) in water, sediment, and fish samples from Ashtoum El-Gamil protected area during May 2010 to January 2011. Data obtained revealed that there were significant (P≤0.0001) differences among sampling seasons and stations as well as their interactions concerning the levels of heavy metals tested in either water, sediment, or fish collected from this protected area. The elements level took the descending order Zn ≥ Cd ≥ Pb ≥ Fe ≥ Cu in the water, Pb ≥ Fe ≥ Cu ≥ Zn ≥ Cd in the sediment, and Fe ≥ Pb ≥ Zn ≥ Cu ≥ Cd in the fish body samples. Proximate analysis of the tested fish (mullet and tilapia) reflected also significant (P≤0.0001) effects due to sampling seasons and stations and their interactions besides fish species. Some significant correlations were calculated among heavy metals (in water, sediments, and fish) and chemical composition of the fish
