A Chromatographic and Electrophoretic Study of Hemoglobin of Domestic Fowl

Abstract

ABSTRACT Gel filtration column chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to study the chemical properties of hemoglobins for 2-week-old broilers, adult broilers and adult layer hen as well as adult ducks. All of the elution patterns obtained with 0.05 M Tris buffer pH 7.4 containing 0.02% sodium azide and monitored at 415 nm for hemoglobin, was consisted of one peak which was estimated as a molecular weight of 51,784 daltons. As compared with the result from SDS-PAGE, the denatured hemoglobin migrated into two bands with the molecular weight of 12,519 daltons and 10,984 daltons