ABSTRACT Stationary phase design in affinity chromatography has to fulfill at least two requirements: non-specific interaction between solid support and or spacer arm and protein target or others is minimal and specific interaction between ligand and protein target is maximal. In this study, stationary phases that consist of polystyrene (PS) as solid support, diaminoalkane as spacer arm and with and without βCD as a ligand are performed in α-amylase separation. Therefore, the research was carried out in two steps; stationary phase preparation and adsorption parameter determination towards α-amylase. Stationary phases that consist of PS, 1,6-diaminohexane and βCD (PS-DAH-βCD) and without βCD (PS-DAH) were synthesized. Stationary phase structure was confirmed by FTIR, , respectively. Due to adsorption parameters, PS-DAP-βCD offer the best adsorption capacity and selectivity as stationary phase in affinity chromatography for α-amylase separation
