Abstract: Lannea barteri (Oliv.) Engl (Anacardiaceae) is a medicinal plant used in west African countries such as Côte d'Ivoire for the treatment of various diseases (wound, rheumatic, diarrhoea). Dichloromethane and methanol extracts from the roots and stem bark of L. barteri were screened for their antibacterial, antifungal, radical scavenging and acetylcholinesterase inhibitory activities. TLC bioautography and agar overlay assay for antifungal activity were run with Cladosporium cucumerinum, Fusarium oxysporum f. sp. vasinfectum, Fusarium oxysporum f. sp. lycopersici and Candida albicans respectively. Also extracts were tested on bacteria (Staphylococcus aureus, Staphylococcus epidermis, Enterococcus faecalis, Proteus mirabilis, Pseudomonas aeruginosa and Escherichia coli), some of which were multidrug resistant bacteria. DPPH and Acetylcholinesterase solutions sprayed on TLC plates were used for radical scavengers and acetylcholinesterase inhibitors. L. barteri gave high positive responses in all four tests, exhibiting activity against bacteria, fungi, free radicals and acetycholinesterase. The phytochemical screening showed that all the extracts contained at least trace amount of steroids, terpenoïds, saponins, quinones, tannins and flavonoïds. This study which is the first report on the biological activities and phytochemicals of Lannea barteri, supports its traditional uses in the treatment of infectious and non infectious diseases
