Abstract: The concept of Synbiotics was implemented to aquaculture feed formulation. Probiotic bacteria were isolated from the locally available confectionary sweetened food. The isolates were characterized through classical biochemical and microbiological techniques to identify them into the group of Lactobacillaceae. Further in the most potent isolate, an antibiotic molecular marker was developed through spontaneous mutation. Mass cultivation of freshwater Spirulina was after isolation and purification. Both were subjected to standardization of mass cultivation and harvesting of biomass. A globulated feed was formulated and fed to aquarium maintained catfish. A conclusion was drawn on the beneficial roles of such Synbiotic culture in Aquaculture nutrition management