In order to select pear cultivar which are genetically compatible with ‘Rocha’ (Sa, Sj), the identification
of S alleles sequences of commercial cultivars with bloom periods overlapping
(partially or totally) the bloom period of ‘Rocha’ was performed. Partial sequences of
the S alleles from the different cultivars were amplified with specific primers, cloned
and sequenced. ‘Pêra De Água’ (S22, Sb) ‘Général Leclerc’ (Sl, Sq) and ‘Alexandrine
Douillard’ (Sb, Sk) should be totally compatible. It was identified a Sj allele in the
cultivars ‘Amêndoa’, ‘Beurré Precoce Morettini’, ‘Clapp’s Rouge’ e ‘Clapp’s
Favourite’ and an allele Sa in ‘Beurré Clairgeau’, indicating that these cultivars are
semi-compatible with ‘Rocha’. ‘Carapinheira’ (Sb) and ‘Pérola’ (Sk) carry a different
allele from the ones of ‘Rocha’ and an amplification pattern completely dissimilar
suggesting full compatibility. In ‘Passe Crassane’ (Sr) and ‘Beurré D’Avril’ (S4), a
different allele from the S alleles of ‘Rocha’ was identified indicating compatibility, but
total or partial compatibility remained unclear, since the amplification pattern does not
exclude that the unknown allele might be Sa from ‘Rocha’
