ABSTRACT The fermentation of sotol juice is mainly a process traditionally carried out by indigenous microorganisms, and knowledge about them allows establishing control parameters during the process. The aim of this study was identified several yeast that participated as starter of stool natural fermentation. Specie identification was performed by 18S rDNA sequencing. Two different yeasts were identified, which had similar 18S rDNA sequences. The two sequences correspond to Kluyveromyces marxianus. The yeasts identified in this study are different from those found in the database