Efficacy of Di-n-octyl Phthalate Anti Venom Isolated from Ceiba pentandra Leaves Extract in Neutralization of Echis ocellatus Venom

Abstract

Abstract: The aim of the study is to investigate the efficacy of Di-n-octyl phthalate isolated from Ceiba pentandra leaves extract for its anti Echis ocellatus venom properties. The leaves of Ceiba pentandra was extracted with aqueous methanol, partitioned on column chromatography and subjected to TLC and preparative TLC. The isolate was tested for its anti venom activity and submitted to IR and GCMS for structural elucidation. Our findings revealed that the isolate was biologically active in inhibiting PLA 2 activity in a dose dependent manner. The IR analysis showed that the isolate has a carbonyl functional group and aromatic ring and the GCMS analysis revealed that the compound isolated was Di-n-octyl phthalate with molecular weight 391. The inhibitor (Di-n-octyl phthalate) isolated during this research might become a highly effective therapeutic agent for the reducing of snake bite envenomation. The relevance of these findings would be of importance in the area of development of anti snake bite drugs that are going to be effective, cheap, accessible and less allergic plant compound that can be scientifically explored