Abstract: A protease enzyme was isolated from maggots, developed from chicken and fish remains. The enzyme was purified by gel filtration on sephadex G-75 followed by ion-exchange chromatography using DEAE-cellulose and was partially characterized. Enzymes activity was found to be 1.87 mmol/unit and 13.34 mmol/unit after each purification stage respectively using casein as substrate. Optimum temperature and pH was 45 °C and 11 respectively. The enzyme has a K m of 0.11 mM and V max of 1.21 mmol/min for casein
