Comparative Studies of the Spectrophotometric and Microbiological Assay Methods Used for the Determination of Ampicillin Levels in Ampicillin/Cloxacillin Combinations
Abstract: The spectrophotometric and microbiological assay methods used for the determination of ampicillin levels in ampicillin/cloxacillin combinations were compared. A total of 24 samples of ampicillin/cloxacillin suspensions from 8 batches and 3 companies (B 2 , D and E) were assayed for their ampicillin content levels using the Spectrophotometric assay method. Infra-red (IR) Spectroscopy confirmed the presence of ampicillin in all the samples and the results obtained showed that the samples had a wide range of amounts of ampicillin which compared favourably with the microbiological assay results of the same products. All the products assayed had ampicillin content within acceptable ranges of the BP and USP. Spectrophotometric assay is recommended for the quick determination of the levels of ampicillin in their combinations with cloxacillin