Abstract.-Freshwater fish, Tor putitora caught from polluted portion of River Kabul was studied for various hepatic biochemical parameters and was compared with control fish caught from non polluted Warsak Dam to know the possible toxic effects of pollution in the river. Fish liver was dissected out and analyzed for various biochemical parameters like total protein, soluble protein, total cholesterol, total lipid, glucose, free amino acid, DNA, RNA and enzymes such as amylase, GOT, GPT and LDH. The hepatic total proteins, soluble proteins, total cholesterol, total lipids, glucose content and free amino acids in fish sample 1 and sample 2 increased 29% and 16%, 6% and 15%, 75% and 68%, 41% and 65%, 47% and 26%, and 333% and 729%, respectively as compared to control sample. The DNA content decreased 14% and 20%, whereas the RNA decreased 41% and 32% in sample 1 and 2, respectively. The fish sample from site I and II showed 5% and 17% increased hepatic amylase activity, 69% and 72% decreased GOT activity in sample 1 and 2, respectively. The GPT activity decreased 45% in sample 1 and increased 28% in sample 2. The LDH activity was decreased 69% in sample 1, but showed 14% increase in sample 2 as compared to control sample. The increase and decrease in various biochemical parameters and enzymes in the liver of test fish samples in comparison with the control shows the adverse effect of aquatic pollution on the fish health. Aquatic pollution could be one of the major reasons of rapid decline in population of this endangered South Asian fresh water fish
