Discriminating 16Sr groups of phytoplasmas by an oligonucleotide microarray targeting 16S-23S spacer region

Abstract

Abstract A microarray detecting phytoplasma 16Sr groups -I, -II, -III, -V, -VI, -X and -XII was developed based on probes targeting 16S-23S spacer region. Despite that it is considered as more suitable than 16S rDNA for the fine differentiation of phytoplasmas, the microarray did not demonstrate discriminatory potential higher than the only phytoplasma microarray published so far based on 16S rDNA. Nevertheless the method used was cheaper and faster bringing the microarray towards routine analysis