International Journal of Farming and Allied Sciences Reduction of negative effects of cefotaxime in tomato transformation by using FeEDDHA

Abstract

ABSTRACT: Tomato (Lycopersicon esculentum) is one of the most important vegetable crops and a genetic model for improving other dicotyledonous crop plants. Agrobacterium mediated gene transformation method is considered as an effective method for transferring foreign genes into plants. cefotaxime, commonly used for elimination of Agrobacterium tumefaciens during plant transformation. In high concentrations of cefotaxime, a decrease in regeneration, reducing the number of shoots and chlorosis were observed. We examined the effect of FeEDDHA on shoot regeneration from cotyledon explants in Falat CH cultivar. When applied during multiplication, FeEDDHA reduced chlorosis, increased the percentage of regeneration and the number of adventitious shoots. Therefore replacement of FeEDTA by FeEDHHA in media reduced the negative effects of cefotaxime