Abstract: A new approach to microcapsule based microbial self-healing system is presented that aims to heal the crack in cementitious materials. In this work, ethyl cellulose (EC) was designed to fabricate microcapsule as a protection strategy to encapsulate an alkaliphilic spore-forming bacterium. The technical feasibility of encapsulated spores and the influence factors were studied by calcium precipitation activity (CPA) of the bacterium. The CPA of broken/unbroken microcapsules was evaluated. The micro-morphology of the precipitation produced by the bacterium was investigated through Environmental Scanning Electron Microscopy (ESEM), X-ray Diffraction (XRD) and X-ray energy dispersive spectroscopy (EDS). X-ray Computed Tomography (XCT) was applied to trace the crack development and self-healing behavior of encapsulated mineralization bacterium in cement paste specimens in three dimensions. The experimental results showed that compared with unbroken microcapsules, higher CPA was achieved by breaking the microcapsule to release the bacterium, suggesting good protection for the encapsulated spores. Subsequent production of calcium carbonate confirmed by ESEM and EDS indicated activation of encapsulated mineralization bacterium. The XCT results showed that formation of crack successfully triggered the breakage of embedded microcapsules. Compared with the specimens without embedded bacterium, the healed crack area of specimens embedded with bacterial microcapsules was monitored, suggesting effective self healing of concrete crack can be achieved by introducing encapsulated mineralization microorganisms into concrete structures
